Resolution of 4-(4-chlorobenzyl)-2-(hexahydro-1-methyl-1H-azepin-4yl)-1(2H)-phthalazinone enantiomers in plasma with frit-FAB LC-MS using a conalbumin column

Nariyasu Mano, Yoshiya Oda, Hiroshi Ohe, Naoki Asakawa, Yutaka Yoshida, Tadashi Sato

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A new column-switching method for analysis of drug enantiomers in plasma has been developed with liquid chromatography-frit fast atom bombardment mass spectrometry in combination with a chiral resolution column, which consists of conalbumin (egg-white glycoprotein) immobilized on silica gel and can be used in the reversed-phase separation mode. This method makes it possible to inject a large volume of deproteinized plasma and obtain resolution of drug enantiomers with high sensitivity. The optimum mobile phase, including a non-volatile buffer such as phosphate buffer, for separation of drugs from a large amount of endogenous compounds can be used, because of inclusion of a trapping column with a desalting function. This method is very simple and rapid, and should be very powerful in studies requiring high-sensitivity analysis with chiral separation of drugs from biological samples such as plasma.

Original languageEnglish
Pages (from-to)557-567
Number of pages11
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume12
Issue number4
DOIs
Publication statusPublished - 1994 Apr
Externally publishedYes

Keywords

  • Frit-FAB LC-MS
  • column switching
  • conalbumin column
  • direct analysis
  • drug enantiomers
  • plasma.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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