Resistin is regulated by C/EBPs, PPARs, and signal-transducing molecules

Haiyan Song, Nobuhiro Shojima, Hideyuki Sakoda, Takehide Ogihara, Midori Fujishiro, Hideki Katagiri, Motonobu Anai, Yukiko Onishi, Hiraku Ono, Kouichi Inukai, Yasushi Fukushima, Masatoshi Kikuchi, Hitoshi Shimano, Nobuhiro Yamada, Yoshitomo Oka, Tomoichiro Asano

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Expression of the adipocyte-derived protein resistin, which is thought to play a key role in the development of insulin resistance in vivo, is regulated by a variety of hormones and mediators, including insulin and TNFα. Here we describe our use of adenovirus-mediated gene transfer to determine which transcription factors and signaling pathways affect resistin expression in 3T3-L1 adipocytes. We found that resistin expression was enhanced by overexpression of C/EBPα and suppressed by C/EBPζ, a negative regulator of C/EBPα. Additionally, C/EBPα induced resistin even in L6 myocytes. Overexpression of PPARγ markedly reduced resistin expressi on, whereas PPARα had no significant effect. Resistin expression was markedly suppressed by overexpression of the PI3-kinase p110α catalytic subunit and by Akt. Finally, overexpression of MEK1, MKK6, or MKK7 suppressed resistin expression. These findings indicate that resistin expression is regulated by C/EBPα and PPARγ, partly via modulation of signal transduction in the PI3-kinase and MAP kinase pathways.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalBiochemical and biophysical research communications
Volume299
Issue number2
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Keywords

  • Adipocyte
  • Adipogenesis PPARs
  • C/EBPs
  • MAP kinase
  • PI3-kinase
  • Resistin
  • Signal transduction
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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