TY - JOUR
T1 - Resistance to pseudorabies virus infection in transgenic mice expressing the chimeric transgene that represses the immediate-early gene transcription
AU - Ono, Etsuro
AU - Tasaki, Takafumi
AU - Kobayashi, Tsutomu
AU - Taharaguchi, Satoshi
AU - Nikami, Hideki
AU - Miyoshi, Ichiro
AU - Kasai, Noriyuki
AU - Arikawa, Jiro
AU - Kida, Hiroshi
AU - Shimizu, Yukio
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture, Japan. We thank Hiroyuki Murota for technical assistance and Dr. T. Minson for providing monoclonal antibody LP1.
PY - 1999/9/15
Y1 - 1999/9/15
N2 - A chimeric gene encoding a fusion protein consisting of the DNA-binding domain of the immediate-early (IE) protein of pseudorabies virus (PRV) and a tail-truncated VP16 of herpes simplex virus 1, lacking the transcription activation domain, has been shown to repress transcription of the PRV IE gene, resulting in the inhibition of PRV growth in vitro. To assess the antiviral potential of the fusion protein in vivo, transgenic mice containing the chimeric gene under the control of the virus-and interferon-inducible Mx 1 promoter were generated. A transgenic mouse line showed marked resistance to PRV infection when the mice were challenged intranasally with PRV. Inhibition of PRV replication was also observed in monolayers of embryonic cells prepared from the transgenic mice. In the cells infected with PRV, transcription of the PRV IE gene was repressed. The present results indicate that the chimeric gene is able to exert a significant antiviral effect against PRV infection in vivo.
AB - A chimeric gene encoding a fusion protein consisting of the DNA-binding domain of the immediate-early (IE) protein of pseudorabies virus (PRV) and a tail-truncated VP16 of herpes simplex virus 1, lacking the transcription activation domain, has been shown to repress transcription of the PRV IE gene, resulting in the inhibition of PRV growth in vitro. To assess the antiviral potential of the fusion protein in vivo, transgenic mice containing the chimeric gene under the control of the virus-and interferon-inducible Mx 1 promoter were generated. A transgenic mouse line showed marked resistance to PRV infection when the mice were challenged intranasally with PRV. Inhibition of PRV replication was also observed in monolayers of embryonic cells prepared from the transgenic mice. In the cells infected with PRV, transcription of the PRV IE gene was repressed. The present results indicate that the chimeric gene is able to exert a significant antiviral effect against PRV infection in vivo.
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U2 - 10.1006/viro.1999.9899
DO - 10.1006/viro.1999.9899
M3 - Article
C2 - 10489342
AN - SCOPUS:0033568091
VL - 262
SP - 72
EP - 78
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -