Resistance to β-lactam antibiotics in Pseudomonas aeruginosa due to interplay between the MexAB-OprM efflux pump and β-lactamase

Taiji Nakae, Akira Nakajima, Toshihisa Ono, Kohjiro Saito, Hiroshi Yoneyama

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

We evaluated the roles of the MexAB-OprM efflux pump and β-lactamase in β-lactam resistance in Pseudomonas aeruginosa by constructing OprM- deficient, OprM basal level, and OprM fully expressed mutants from β- lactamase-negative, -inducible, and -overexpressed strains. We conclude that, with the notable exception of imipenem, the MexAB-OprM pump contributes significantly to β-lactam resistance in both β-lactamase-negative and β- lactamase-inducible strains, while the contribution of the MexAB-OprM efflux system is negligible in strains with overexpressed β-lactamase. Overexpression of the efflux pump alone contributes to the high level of β- lactam resistance in the absence of β-lactamase.

Original languageEnglish
Pages (from-to)1301-1303
Number of pages3
JournalAntimicrobial agents and chemotherapy
Volume43
Issue number5
DOIs
Publication statusPublished - 1999 May
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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