Residue aspartate-147 from the third transmembrane region of Na+/H+ antiporter NhaB of Vibrio alginolyticus plays a role in its activity

T. Nakamura, Y. Fujisaki, H. Enomoto, Y. Nakayama, T. Takabe, N. Yamaguchi, N. Uozumi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

NhaB is a bacterial Na+/H+ antiporter with unique topology. The pH dependence of NhaB from Vibrio alginolyticus differs from that of the Escherichia coli NhaB homolog. Replacement of Asp-147 with Glu made high H+ concentrations a requirement for the NhaB activity. Replacement of Asp-147 with neutral amino acids inactivated NhaB.

Original languageEnglish
Pages (from-to)5762-5767
Number of pages6
JournalJournal of bacteriology
Volume183
Issue number19
DOIs
Publication statusPublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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