Requirement of Negative Residues, Asp 95 and Asp 105, in S2 on Membrane Integration of a Voltage-dependent K+ Channel, KAT1

Yoko Sato, Yoshihiro Hosoo, Masao Sakaguchi, Nobuyuki Uozumi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Voltage-dependent K+ channels consist of a voltage-sensing region and a pore-forming region. Here we have identified the negative residues of the second transmembrane segment in the plant voltage-dependent K+ channel, KAT1, which involves the function of voltage sensing. Point mutations at D95 and D105 but not D89 and D116 failed to complement the K+ uptake deficient properties of the mutant yeast. In vitro translation and translocation experiments showed that the membrane integration of the third and fourth segments involving voltage sensor were impaired by the replacement of D95 or D105 by serine. These data show that both the residues play a crucial role in the membrane topogenesis of the voltage sensor in KAT1.

Original languageEnglish
Pages (from-to)923-926
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number4
DOIs
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Keywords

  • Channel
  • Potassium
  • Topogenesis
  • Topology
  • Transmembrane

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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