Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

Hiroaki Nagatomo, Nanami Kohri, Hiroki Akizawa, Yumi Hoshino, Nobuhiko Yamauchi, Tomohiro Kono, Masashi Takahashi, Manabu Kawahara

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.

    Original languageEnglish
    Pages (from-to)457-461
    Number of pages5
    JournalAnimal Science Journal
    Volume87
    Issue number3
    DOIs
    Publication statusPublished - 2016 Mar 1

    Keywords

    • NASP
    • bovine embryo
    • knockdown

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)

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  • Cite this

    Nagatomo, H., Kohri, N., Akizawa, H., Hoshino, Y., Yamauchi, N., Kono, T., Takahashi, M., & Kawahara, M. (2016). Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development. Animal Science Journal, 87(3), 457-461. https://doi.org/10.1111/asj.12538