Repression of the K+ uptake and cation-stimulated ATPase activity associated with the plasma membrane-enriched fraction of cucumber roots due to Ca2+ starvation

Hideaki Matsumoto, Tomoyuki Yamaya

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The uptake of K+ by cucumber plants decreased markedly during Ca2+ starvation. A plasma membrane-enriched fraction, judged from the distribution of marker enzymes, was prepared from control and Ca2+-starved roots. The Mg2+- and K+-Mg2+-ATPase activities associated with the plasma membrane-enriched fraction of control roots were maxima at pH 6.5. Various monovalent cations and potassium salts of monovalent anions stimulated Mg2+-ATPase activity. Vanadate, DES and DCCD inhibited K+- Mg2+-ATPase activity. Of the divalent cations and phosphate esters tested, Mg2+ and ATP were most effective for the stimulation of ATPase by K+, whereas Ca2+ was ineffective in replacing Mg2+.Mg2+- and K+-Mg2+-ATPase activities associated with the plasma membrane enriched fraction of Ca2+-starved roots were much lower than those of control roots. Km values of K+-Mg2+-ATPase for ATP were comparable for control and Ca2+-starved roots.The K+-stimulated activity of Mg2+-ATPase in Ca2+-starved roots was approximately one fourth that of the control, whereas the rate of stimulation was only slightly lower in Ca2+-starved roots.

Original languageEnglish
Pages (from-to)1501-1511
Number of pages11
JournalPlant and Cell Physiology
Volume25
Issue number8
DOIs
Publication statusPublished - 1984 Dec
Externally publishedYes

Keywords

  • Cucumber
  • Plasma membrane ATPase
  • Repression

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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