Repetitive short-pulse light mainly inactivates photosystem i in sunflower leaves

Takehiro Sejima, Daisuke Takagi, Hiroshi Fukayama, Amane Makino, Chikahiro Miyake

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

Under field conditions, the leaves of plants are exposed to fluctuating light, as observed in sunfleck. The duration and frequency of sunfleck, which is caused by the canopy being blown by the wind, are in the ranges from 0.2 to 50 s, and from 0.004 to 1 Hz, respectively. Furthermore, >60% of the sunfleck duration ranges from 0.2 to 0.8 s. In the present research, we analyzed the effects of repetitive illumination by short-pulse (SP) light of sunflower leaves on the photosynthetic electron flow. The duration of SP light was set in the range from 10 to 300 ms. We found that repetitive illumination with SP light did not induce the oxidation of P700 in PSI, and mainly inactivated PSI. Increases in the intensity, duration and frequency of SP light enhanced PSI photoinhibition. PSI photoinhibition required the presence of O2. The inactivation of PSI suppressed the net CO2 assimilation. On the other hand, the increase in the oxidized state of P700 suppressed PSI inactivation. That is, PSI with a reduced reaction center would produce reactive oxygen species (ROS) by SP light, leading to PSI photodamage. This mechanism probably explains the PSI photodamage induced by constant light.

Original languageEnglish
Pages (from-to)1184-1193
Number of pages10
JournalPlant and Cell Physiology
Volume55
Issue number6
DOIs
Publication statusPublished - 2014 Jun

Keywords

  • Photoinhibition
  • Photosystem I
  • Reactive oxygen species (ROS)
  • Water-Water Cycle (WWC)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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