TY - JOUR
T1 - Repair of apurinic/apyrimidinic sites by UV damage endonuclease; a repair protein for UV and oxidative damage
AU - Kanno, Shin Ichiro
AU - Iwai, Shigenori
AU - Takao, Masashi
AU - Yasui, Akira
N1 - Funding Information:
We thank Dr S. McCready for reading the text and for valuable comments. We thank Mr Y. Inoue and Mr H. Sasaki for making antibodies. This work was supported by grants (08280101 and JSPS-RFTF 97L00501) to A.Y.
PY - 1999/8/1
Y1 - 1999/8/1
N2 - UV damage endonuclease (UVDE) initiates a novel form of excision repair by introducing a nick immediately 5' to UV-induced cyclobutane pyrimidine dimers or 6-4 photoproducts. Here, we report that apurinic/apyrimidinic (AP) sites are also nicked by Neurospora crassa and Schizosaccharomyces pombe UVDE. UVDE introduces a nick immediately 5' to the AP site leaving a 3'-OH and a 5'-phosphate AP. Apyrimidinic sites are more effectively nicked by UVDE than apurinic sites. UVDE also possesses 3'-repair activities for AP sites nicked by AP lyase and for 3'-phosphoglycolate produced by bleomycin. The Uvde gene introduced into Escherichia coli cells lacking two types of AP endonuclease, Exo III and Endo IV, gave the host cells resistance to methyl-methane sulfonate and t-butyl hydroperoxide. We identified two AP endonuclease activities in S.pombe cell extracts. Besides cyclobutane pyrimidine dimers and 6-4 photoproducts, N.crassa UVDE also nicks Dewar photoproducts. Thus, UVDE is able to repair both of the major forms of DNA damage in living organisms: UV-induced DNA lesions and AP sites.
AB - UV damage endonuclease (UVDE) initiates a novel form of excision repair by introducing a nick immediately 5' to UV-induced cyclobutane pyrimidine dimers or 6-4 photoproducts. Here, we report that apurinic/apyrimidinic (AP) sites are also nicked by Neurospora crassa and Schizosaccharomyces pombe UVDE. UVDE introduces a nick immediately 5' to the AP site leaving a 3'-OH and a 5'-phosphate AP. Apyrimidinic sites are more effectively nicked by UVDE than apurinic sites. UVDE also possesses 3'-repair activities for AP sites nicked by AP lyase and for 3'-phosphoglycolate produced by bleomycin. The Uvde gene introduced into Escherichia coli cells lacking two types of AP endonuclease, Exo III and Endo IV, gave the host cells resistance to methyl-methane sulfonate and t-butyl hydroperoxide. We identified two AP endonuclease activities in S.pombe cell extracts. Besides cyclobutane pyrimidine dimers and 6-4 photoproducts, N.crassa UVDE also nicks Dewar photoproducts. Thus, UVDE is able to repair both of the major forms of DNA damage in living organisms: UV-induced DNA lesions and AP sites.
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U2 - 10.1093/nar/27.15.3096
DO - 10.1093/nar/27.15.3096
M3 - Article
C2 - 10454605
AN - SCOPUS:0033178699
VL - 27
SP - 3096
EP - 3103
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 15
ER -