TY - JOUR
T1 - Renin release from isolated afferent arterioles
AU - Itoh, S.
AU - Carretero, O. A.
AU - Murray, R. D.
N1 - Funding Information:
the Federation of American Societies for Experimental Biology, St. Louis, Missouri, USA, 1984, and published in abstract form (Fed Proc 43:1076, 1984). The study was supported by National Institutes of Health grants HL28982 and HL15839. The authors thank A. Cheng for technical assistance.
PY - 1985
Y1 - 1985
N2 - A new in vitro system was developed in which renin release was studied in the absence of tubules, glomeruli, and macula densa. Rabbit afferent arterioles were isolated by microdissection and incubated in medium 199 for consecutive 15-min periods. Renin concentration of incubation media and arteriolar tissue was measured using partially purified rabbit angiotensinogen. Basal renin release rate was 0.68 ± 0.06 ng of angiotensin I (AI)·hr-1·arteriole-1/h4 incubation of arterioles (x̄ ± SEM, N = 29), and remained stable for 60 min. The renin release rate was 2.76 ± 0.22% of arteriolar renin content each hour, and there was a significant correlation between the two (r = 0.73, P < 0.01). Renin release increased from 0.56 ± 0.07 to 1.71 ± 0.17 ngAI·hr-1·arteriole-1/hr (P < 0.01, N = 6) during exposure to isoproterenol (8.1 x 10-5 M) and returned to basal values during the recovery period. Dietary sodium depletion resulted in a significantly greater arteriolar renin content (86.1 ± 17.5 ngAI·hr-1/arteriole) compared with that from rabbits on a normal sodium diet (26.8 ± 2.51 ngAI·hr-1/arteriole). However, sodium depletion did not alter the basal renin release rate suggesting that sodium depletion increased renin content in a storage pool rather than a pool contributing to basal release. It is concluded that the isolated afferent arteriole is a good model for the study of renin release in the absence of tubules, glomeruli, and macula densa.
AB - A new in vitro system was developed in which renin release was studied in the absence of tubules, glomeruli, and macula densa. Rabbit afferent arterioles were isolated by microdissection and incubated in medium 199 for consecutive 15-min periods. Renin concentration of incubation media and arteriolar tissue was measured using partially purified rabbit angiotensinogen. Basal renin release rate was 0.68 ± 0.06 ng of angiotensin I (AI)·hr-1·arteriole-1/h4 incubation of arterioles (x̄ ± SEM, N = 29), and remained stable for 60 min. The renin release rate was 2.76 ± 0.22% of arteriolar renin content each hour, and there was a significant correlation between the two (r = 0.73, P < 0.01). Renin release increased from 0.56 ± 0.07 to 1.71 ± 0.17 ngAI·hr-1·arteriole-1/hr (P < 0.01, N = 6) during exposure to isoproterenol (8.1 x 10-5 M) and returned to basal values during the recovery period. Dietary sodium depletion resulted in a significantly greater arteriolar renin content (86.1 ± 17.5 ngAI·hr-1/arteriole) compared with that from rabbits on a normal sodium diet (26.8 ± 2.51 ngAI·hr-1/arteriole). However, sodium depletion did not alter the basal renin release rate suggesting that sodium depletion increased renin content in a storage pool rather than a pool contributing to basal release. It is concluded that the isolated afferent arteriole is a good model for the study of renin release in the absence of tubules, glomeruli, and macula densa.
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U2 - 10.1038/ki.1985.77
DO - 10.1038/ki.1985.77
M3 - Article
C2 - 3894761
AN - SCOPUS:0021802673
VL - 27
SP - 762
EP - 767
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 5
ER -