Renaturation and activation of calf prochymosin produced in an insoluble form in Escherichia coli

Yoshiyuki Kawaguchi, Norio Shimizu, Katsuhiko Nishimori, Takeshi Uozumi, Teruhiko Beppu

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Calf prochymosin produced in Escherichia coli cells harboring the expression plasmids was insoluble and formed large inclusion bodies, which were solubilized by 8 M urea. The conditions allowing correct refolding of denatured prochymosin were investigated. Dialysis at pH 10 in the presence of 500 mM NaCl was found to give the maximum renaturation, and subsequent acidic treatment for autocatalytic processing of refolded prochymosin allowed almost 100% recovery of chymosin.

Original languageEnglish
Pages (from-to)307-315
Number of pages9
JournalJournal of Biotechnology
Volume1
Issue number5-6
DOIs
Publication statusPublished - 1984 Dec

Keywords

  • chymosin
  • prochymosin
  • renaturation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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