TY - JOUR
T1 - Renal expression of the essential genes associated with sodium transport following a total proctocolectomy in rats
AU - Fukushima, Kouhei
AU - Sato, Shun
AU - Naito, Hiroo
AU - Funayama, Yuji
AU - Shibata, Chikashi
AU - Sasaki, Iwao
PY - 2005/6
Y1 - 2005/6
N2 - The water and electrolyte balance is regulated by the renin-angiotensin- aldosterone system in the kidney. We previously reported that the levels of circulating aldosterone dramatically increased following a total proctocolectomy in rats. However, there is no direct evidence regarding whether renal adaptation is accelerated by the induction of aldosterone-associated molecules. To explore this question, Sprague-Dawley rats underwent total proctocolectomies and then were killed 8 weeks later. We investigated the renal expression of 11β-hydroxysteroid dehydrogenase type 2, the α-, β-, and γ-subunits of the epithelial sodium channel, and the α1- and β1-subunits of Na+,K+-ATPase mRNAs because those molecules are responsible for the aldosterone specificity for mineralocorticoid receptor, amiloride-sensitive sodium absorption, and sodium extrusion from distal tubules, respectively. A Northern blot analysis demonstrated the kidney to exhibit mRNA induction for all of these molecules, thus supporting the idea that renal adaptation following a total proctocolectomy depends, at least in part, upon the molecular induction which is principally regulated by circulating aldosterone.
AB - The water and electrolyte balance is regulated by the renin-angiotensin- aldosterone system in the kidney. We previously reported that the levels of circulating aldosterone dramatically increased following a total proctocolectomy in rats. However, there is no direct evidence regarding whether renal adaptation is accelerated by the induction of aldosterone-associated molecules. To explore this question, Sprague-Dawley rats underwent total proctocolectomies and then were killed 8 weeks later. We investigated the renal expression of 11β-hydroxysteroid dehydrogenase type 2, the α-, β-, and γ-subunits of the epithelial sodium channel, and the α1- and β1-subunits of Na+,K+-ATPase mRNAs because those molecules are responsible for the aldosterone specificity for mineralocorticoid receptor, amiloride-sensitive sodium absorption, and sodium extrusion from distal tubules, respectively. A Northern blot analysis demonstrated the kidney to exhibit mRNA induction for all of these molecules, thus supporting the idea that renal adaptation following a total proctocolectomy depends, at least in part, upon the molecular induction which is principally regulated by circulating aldosterone.
KW - Aldosterone
KW - Renal compensation
KW - Total proctocolectomy
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U2 - 10.1007/s00595-004-2971-6
DO - 10.1007/s00595-004-2971-6
M3 - Article
C2 - 15912302
AN - SCOPUS:21044456016
VL - 35
SP - 502
EP - 504
JO - Surgery Today
JF - Surgery Today
SN - 0941-1291
IS - 6
ER -