Release of mitochondrial cytochrome c and DNA fragmentation after cold injury-induced brain trauma in mice: Possible role in neuronal apoptosis

Yuiko Morita-Fujimura, Miki Fujimura, Makoto Kawase, Sylvia F. Chen, Pak H. Chan

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Recent studies have shown that release of mitochondrial cytochrome c is a critical step in the apoptosis process. In this study, we examined the subcellular distribution of the cytochrome c protein after cold injury (CI), in which apoptosis is assumed to participate. Western blotting and immunohistochemistry showed cytosolic cytochrome c as early as 1 h after CI, and correspondingly, there was a reduction in mitochondrial cytochrome c after injury. Neuronal distribution of cytosolic cytochrome c was shown by double staining with a neuronal nuclear marker by immunohistochemistry. A significant amount of DNA laddering was detected 4 h after CI, and increased in a time-dependent manner. These data suggest that early cytochrome c release from mitochondria may contribute to apoptosis induction after traumatic brain injury.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalNeuroscience Letters
Volume267
Issue number3
DOIs
Publication statusPublished - 1999 Jun 4

Keywords

  • Apoptosis
  • Caspase
  • Cold injury
  • Cytochrome c
  • Mitochondrial injury
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)

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