Release of mitochondrial apoptogenic factors and cell death are mediated by CK2 and NADPH oxidase

Gab Seok Kim, Joo Eun Jung, Purnima Narasimhan, Hiroyuki Sakata, Hideyuki Yoshioka, Yun Seon Song, Nobuya Okami, Pak H. Chan

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Activation of the NADPH oxidase subunit, NOX2, and increased oxidative stress are associated with neuronal death after cerebral ischemia and reperfusion. Inhibition of NOX2 by casein kinase 2 (CK2) leads to neuronal survival, but the mechanism is unknown. In this study, we show that in copper/zinc-superoxide dismutase transgenic (SOD1 Tg) mice, degradation of CK2α and CK2α′ and dephosphorylation of CK2Β against oxidative stress were markedly reduced compared with wild-type (WT) mice that underwent middle cerebral artery occlusion. Inhibition of CK2 pharmacologically or by ischemic reperfusion facilitated accumulation of poly(ADP-ribose) polymers, the translocation of apoptosis-inducing factor (AIF), and cytochrome c release from mitochondria after ischemic injury. The eventual enhancement of CK2 inhibition under ischemic injury strongly increased 8-hydroxy-2′- deoxyguanosine and phosphorylation of H2A.X. Furthermore, CK2 inhibition by tetrabromocinnamic acid (TBCA) in SOD1 Tg and gp91 knockout (KO) mice after ischemia reperfusion induced less release of AIF and cytochrome c than in TBCA-treated WT mice. Inhibition of CK2 in gp91 KO mice subjected to ischemia reperfusion did not increase brain infarction compared with TBCA-treated WT mice. These results strongly suggest that NOX2 activation releases reactive oxygen species after CK2 inhibition, triggering release of apoptogenic factors from mitochondria and inducing DNA damage after ischemic brain injury.

Original languageEnglish
Pages (from-to)720-730
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume32
Issue number4
DOIs
Publication statusPublished - 2012 Apr

Keywords

  • Casein kinase 2
  • NADPH oxidase
  • NOX2
  • middle cerebral artery occlusion
  • reactive oxygen species

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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