Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition

Takahiro Henmi; Mitsue Miyao; Yasusi Yamamoto

doi:10.1093/pcp/pch027

Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition

Takahiro Henmi, Mitsue Miyao, Yasusi Yamamoto

AGR - Life Sciences

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.

Original language	English
Pages (from-to)	243-250
Number of pages	8
Journal	Plant and Cell Physiology
Volume	45
Issue number	2
DOIs	https://doi.org/10.1093/pcp/pch027
Publication status	Published - 2004 Feb

Keywords

OEC subunits
Photodamage
Photoinhibition
Photosystem II
Reactive oxygen species
Spinach

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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10.1093/pcp/pch027

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title = "Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition",

abstract = "Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.",

keywords = "OEC subunits, Photodamage, Photoinhibition, Photosystem II, Reactive oxygen species, Spinach",

author = "Takahiro Henmi and Mitsue Miyao and Yasusi Yamamoto",

note = "Funding Information: This work was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan to Y.Y. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Henmi, Takahiro

AU - Miyao, Mitsue

AU - Yamamoto, Yasusi

N1 - Funding Information: This work was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan to Y.Y. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

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AB - Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.

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