Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition

Takahiro Henmi, Mitsue Miyao, Yasusi Yamamoto

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalPlant and Cell Physiology
Volume45
Issue number2
DOIs
Publication statusPublished - 2004 Feb

Keywords

  • OEC subunits
  • Photodamage
  • Photoinhibition
  • Photosystem II
  • Reactive oxygen species
  • Spinach

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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