TY - JOUR
T1 - Relationship between reservoir size and genetic differentiation of the stream caddisfly Stenopsyche marmorata
AU - Watanabe, Kozo
AU - Omura, Tatsuo
N1 - Funding Information:
This research was partially supported by the Ministry of Education, Science, Sports and Culture and the Japan Society for the Promotion of Science (JSPS). We also give our thanks to Dr. J. Yokoyama for teaching us DNA experiments.
PY - 2007/4
Y1 - 2007/4
N2 - Reservoirs have the potential to block the gene flow of stream macroinvertebrates along a channel, which takes place via larval drift and adult flight, resulting in genetic differentiation above and below the reservoirs. Gene flow across the reservoirs may be strongly obstructed if the area of standing water is larger. Using random amplified polymorphic DNA markers, we investigated the genetic structure of Stenopsyche marmorata (Stenopsychidae: Trichoptera) populations found above and below the reservoirs, and the reference stream of six reservoirs with small to large water surface area ranging from 0.12 km2 to 6.00 km2. As a result, the two largest reservoirs with a standing water area larger than 3.27 km2 showed significant differences in pairwise θ between fragmented and reference streams, whereas the other four reservoirs with a standing water area smaller than 1.64 km2 showed insignificant differences. The genetic differentiations in the two largest reservoirs did not result in the reduction of genetic diversities in the fragments. Based on the significant correlation between relative population size and mean expected heterozygosity, we concluded that local genetic diversities were constrained in smaller populations due to the effect of genetic drift.
AB - Reservoirs have the potential to block the gene flow of stream macroinvertebrates along a channel, which takes place via larval drift and adult flight, resulting in genetic differentiation above and below the reservoirs. Gene flow across the reservoirs may be strongly obstructed if the area of standing water is larger. Using random amplified polymorphic DNA markers, we investigated the genetic structure of Stenopsyche marmorata (Stenopsychidae: Trichoptera) populations found above and below the reservoirs, and the reference stream of six reservoirs with small to large water surface area ranging from 0.12 km2 to 6.00 km2. As a result, the two largest reservoirs with a standing water area larger than 3.27 km2 showed significant differences in pairwise θ between fragmented and reference streams, whereas the other four reservoirs with a standing water area smaller than 1.64 km2 showed insignificant differences. The genetic differentiations in the two largest reservoirs did not result in the reduction of genetic diversities in the fragments. Based on the significant correlation between relative population size and mean expected heterozygosity, we concluded that local genetic diversities were constrained in smaller populations due to the effect of genetic drift.
KW - Gene flow
KW - Genetic diversity
KW - Habitat fragmentation
KW - Population size
KW - RAPD
UR - http://www.scopus.com/inward/record.url?scp=33847292334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847292334&partnerID=8YFLogxK
U2 - 10.1016/j.biocon.2006.11.014
DO - 10.1016/j.biocon.2006.11.014
M3 - Article
AN - SCOPUS:33847292334
VL - 136
SP - 203
EP - 211
JO - Biological Conservation
JF - Biological Conservation
SN - 0006-3207
IS - 2
ER -