TY - JOUR
T1 - Relationship between O2 evolution capacity and cytochrome b-559 high-potential form in Photosystem II particles
AU - Briantais, Jean Marie
AU - Vernotte, Claudie
AU - Miyao, Mitsue
AU - Murata, Norio
AU - Picaud, Martine
PY - 1985/7/17
Y1 - 1985/7/17
N2 - As previously shown for inside-out vesicles by Larsson et al. (Larsson, C., Jansson, C., Ljungberg, U.L., Åkerlund, H.E. and Anderson, B. (1984) in Advances in Photosynthesis Research, Vol. I, pp. 363-366 (Sybesma C., ed.), Martinus Nijhoff/Dr. W. Junk Publishers, Dordrecht, The Netherlands), we observed that NaCl 1 M washing of Photosystem II particles prepared by Triton X-100 treatment of spinach thylakoids induces both an inactivation of oxygen evolution and transformation of cytochrome b-559 from its high-potential to its low-potential form. A partial reactivation of water oxidation by 24 kDa polypeptide refixation is accompanied by a partial restoration of the cytochrome b-559 high-potential (HP) form. In contrast, reconstitution of water splitting by Ca2+ addition is not associated to a reestablishment of the cytochrome (HP) form. We conclude that cytochrome b-559 HP plays no role in water oxidation.
AB - As previously shown for inside-out vesicles by Larsson et al. (Larsson, C., Jansson, C., Ljungberg, U.L., Åkerlund, H.E. and Anderson, B. (1984) in Advances in Photosynthesis Research, Vol. I, pp. 363-366 (Sybesma C., ed.), Martinus Nijhoff/Dr. W. Junk Publishers, Dordrecht, The Netherlands), we observed that NaCl 1 M washing of Photosystem II particles prepared by Triton X-100 treatment of spinach thylakoids induces both an inactivation of oxygen evolution and transformation of cytochrome b-559 from its high-potential to its low-potential form. A partial reactivation of water oxidation by 24 kDa polypeptide refixation is accompanied by a partial restoration of the cytochrome b-559 high-potential (HP) form. In contrast, reconstitution of water splitting by Ca2+ addition is not associated to a reestablishment of the cytochrome (HP) form. We conclude that cytochrome b-559 HP plays no role in water oxidation.
KW - (Spinach chloroplast)
KW - Cytochrome b-559
KW - Oxygen evolution
KW - Photosystem II
KW - Water splitting
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U2 - 10.1016/0005-2728(85)90019-2
DO - 10.1016/0005-2728(85)90019-2
M3 - Article
AN - SCOPUS:0010229578
VL - 808
SP - 348
EP - 351
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 2
ER -