TY - JOUR
T1 - Relationship between Molecular State of Serum HBV-DNA and Clinical Features of Hepatitis B Virus Carriers
AU - Sato, Shuichi
AU - Yamada, Miki
AU - Miyazaki, Yutaka
AU - Matsuda, Keizaburoh
AU - Kanno, Atsushi
AU - Ishii, Motoyasu
AU - Ohori, Hitoshi
AU - Toyota, Takayoshi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - Molecular species of serum hepatitis B virus (HBV)-DNA in HBV carriers were classified by Southern blot hybridization into three types; type I with two bands of 4.0 kb and 3.2 kb, type II with the two bands of type I plus the smear between 4.0 kb and 3.2 kb, and type III with a broad band below 4.0 kb. A total of 51 HBV carriers were classified into three groups (group I, n=19 with type I; group II, n=12 with type II; and group III, n=20 with type III). Serum aminotransferase levels of group I were significantly lower than those of groups II and III. Liver pathology revealed that 18 of the 19 (94.7%) group I patients showed nonspecific reactive hepatitis (NSRH), while 11 of the 12 (91.7%) group II patients and 19 of the 20 (95.0%) group III patients showed chronic persistent hepatitis (CPH) or chronic active hepatitis (CAH). Immunohistochemical study showed that hepatitis B core antigen (HBcAg) was localized in the nucleus of hepatocytes in most of patients with type I while it was localized in both the nucleus and cytoplasm in those with types II and III. Since the smear between 4.0 kb and 3.2 kb is specifically found in groups II and III, HBV-DNA with this smear may be related to active hepatitis.
AB - Molecular species of serum hepatitis B virus (HBV)-DNA in HBV carriers were classified by Southern blot hybridization into three types; type I with two bands of 4.0 kb and 3.2 kb, type II with the two bands of type I plus the smear between 4.0 kb and 3.2 kb, and type III with a broad band below 4.0 kb. A total of 51 HBV carriers were classified into three groups (group I, n=19 with type I; group II, n=12 with type II; and group III, n=20 with type III). Serum aminotransferase levels of group I were significantly lower than those of groups II and III. Liver pathology revealed that 18 of the 19 (94.7%) group I patients showed nonspecific reactive hepatitis (NSRH), while 11 of the 12 (91.7%) group II patients and 19 of the 20 (95.0%) group III patients showed chronic persistent hepatitis (CPH) or chronic active hepatitis (CAH). Immunohistochemical study showed that hepatitis B core antigen (HBcAg) was localized in the nucleus of hepatocytes in most of patients with type I while it was localized in both the nucleus and cytoplasm in those with types II and III. Since the smear between 4.0 kb and 3.2 kb is specifically found in groups II and III, HBV-DNA with this smear may be related to active hepatitis.
KW - HBV carriers
KW - HBV-DNA
KW - HBcAg
KW - Southern blot hybridization
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U2 - 10.1620/tjem.171.309
DO - 10.1620/tjem.171.309
M3 - Article
C2 - 8184405
AN - SCOPUS:0027831771
VL - 171
SP - 309
EP - 317
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
SN - 0040-8727
IS - 4
ER -