Relationship between glucocorticoid receptor and response to glucocorticoid therapy in ulcerative colitis

Takenobu Shimada, Nobuo Hiwatashi, Hideo Yamazaki, Yoshitaka Kinouchi, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

PURPOSE: To clarify the relationship between the glucocorticoid receptor and the effectiveness of glucocorticoid therapy in patients with ulcerative colitis, we investigated the number and apparent dissociation constant of glucocorticoid receptor in peripheral blood mononuclear leukocytes of patients with ulcerative colitis. MATERIALS AND METHODS: Eleven patients with ulcerative colitis (5 who responded to intravenous glucocorticoids and 6 who did not) and ten control subjects were studied. The number and apparent dissociation constant of glucocorticoid receptor were measured using a whole-cell binding assay. Results were expressed as a median (interquartile range). RESULTS: The number of glucocorticoid receptors from the six non-responders, five responders, and ten healthy controls were 4922 (range, 4484-5643), 3413 (range, 3183-4450), and 3610 (range, 2594-3979) binding sites/cell, respectively. The apparent dissociation constant of the glucocorticoid receptors from the nonresponders, responders, and healthy controls were 7.03 (range, 5.66-10), 4.27 (range, 4-5.13), and 6.18 (range, 5.86-6.74) nM, respectively. Nonresponders had a significant increase both in the number of binding sites and in the apparent dissociation constant compared with responders (P = 0.045; P = 0.029). CONCLUSIONS: The increased number and apparent dissociation constant of glucocorticoid receptor are closely associated with the effectiveness of glucocorticoid therapy. The measurement of the number and apparent dissociation constant of glucocorticoid receptor may be useful in predicting response to glucocorticoids.

Original languageEnglish
Pages (from-to)S54-S58
JournalDiseases of the Colon and Rectum
Volume40
Issue number10 SUPPL.
DOIs
Publication statusPublished - 1997

Keywords

  • Glucocorticoid receptor
  • Glucocorticoid resistance
  • Ulcerative colitis
  • Whole-cell binding assay

ASJC Scopus subject areas

  • Gastroenterology

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