Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film

Sadaaki Yamamoto, Masaru Tanaka, Hiroshi Sunami, Keiko Arai, Aiko Takayama, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura

    Research output: Contribution to journalArticlepeer-review

    48 Citations (Scopus)


    Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(ε-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film.

    Original languageEnglish
    Pages (from-to)3785-3791
    Number of pages7
    JournalSurface Science
    Issue number18
    Publication statusPublished - 2006 Sep 15


    • Adsorption
    • Atomic force microscopy
    • Cell adhesion
    • Fibronectin
    • Scanning electron microscopy
    • Self-assembly
    • Surface topography
    • Tissue engineering

    ASJC Scopus subject areas

    • Condensed Matter Physics
    • Surfaces and Interfaces
    • Surfaces, Coatings and Films
    • Materials Chemistry

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