Relatedness structure estimated by microsatellites DNA and mitochondrial DNA polymerase chain reaction - Restriction fragment length polymorphisms analyses in the wild population of kuruma prawn Penaeus japonicus

Takuma Sugaya, Minoru Ikeda, Nobuhiko Taniguchi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The genetic structure and variability of four wild populations of kuruma prawn in Japan were examined by estimating relatedness among individuals. The relatedness was estimated by five microsatellites (MS) DNA markers. Examination of relatedness showed that individuals were related significantly in Kumamoto and Kagoshima. In Kagoshima, some individuals showed full-sib level of relatedness. The analysis of mitochondrial (mt)DNA polymerase chain reaction - restriction fragment length polymorphisms (PCR-RFLP) was also performed, showing that the closely related individuals in Kagoshima tended to share a common haplotype. It is, therefore, supposed that there are many kins in Kumamoto and Kagoshima. However, the heterozygosities and allelic and genotypic frequencies in MS-DNA analysis were not significantly different among the localities. Moreover, the haplotype distributions of mtDNA in Kumamoto and Kagoshima were significantly different from other localities. Thus, it is suggested that no spatial differentiations occurred due to the geographic or historical effects between the localities and that there is the possibility of a mixture of hatchery populations in Kumamoto and Kagoshima.

Original languageEnglish
Pages (from-to)793-802
Number of pages10
JournalFisheries Science
Volume68
Issue number4
DOIs
Publication statusPublished - 2002 Aug 1

Keywords

  • Genetic relatedness
  • Kuruma prawn
  • Microsatellite DNA
  • Mitochondrial DNA
  • Penaeus japonicus
  • Polymerase chain reaction - restriction fragment length polymorphisms

ASJC Scopus subject areas

  • Aquatic Science

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