TY - JOUR
T1 - REI-1 Is a Guanine Nucleotide Exchange Factor Regulating RAB-11 Localization and Function in C. elegans Embryos
AU - Sakaguchi, Aisa
AU - Sato, Miyuki
AU - Sato, Katsuya
AU - Gengyo-Ando, Keiko
AU - Yorimitsu, Tomohiro
AU - Nakai, Junichi
AU - Hara, Taichi
AU - Sato, Ken
AU - Sato, Ken
N1 - Funding Information:
We thank I. Maejima, H. Kobayashi, T. Akuzawa, R. Hirai, H. Ooyama, and S. Inoue for technical assistance; S. Kasuga and Y. Kagawa-Nagamura for technical assistance in isolating rei-1 and rei-2 knockout mutants; B. Grant and A. Audhya for reagents and strains; A. Spang for an antibody; C. Frøkjaer-Jensen and S. Ahmed for plasmids; and N. Hisamoto, K. Matsumoto, F. Tokunaga, S. Yoshida, A. Harada, S. Yoshimura, and Ken Sato (Gunma University) lab members for technical advice. Some strains used in this work were provided by the Caenorhabditis Genetics Center supported by the NIH Office of Research Infrastructure Programs P40 OD010440. This research was supported by the JSPS Kakenhi 25870119 (A.S.); JSPS Kakenhi 23687027, MEXT Kakenhi 26111503, and Uehara Memorial Foundation (M.S.); JSPS Kakenhi 22500353 (K.G.-A.) and Regional Innovation Cluster Program (City Area Type, Central Saitama Area) (J.N. and K.G.-A.); and JSPS Kakenhi 24590341 (T.H.). It was also supported in part by the JSPS Kakenhi 25440079 and MEXT Kakenhi 25117502 and 26102509 (K.S., University of Tokyo) and by the JSPS Kakenhi 26291036, Sumitomo Foundation, Naito Foundation, and Mochida Memorial Foundation for Medical and Pharmaceutical Research, as well as by a research fellowship from Gunma University (K.S., Gunma University). This work was also supported by the Joint Research Program of the Institute for Molecular and Cellular Regulation at Gunma University.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/10/26
Y1 - 2015/10/26
N2 - The small GTPase Rab11 dynamically changes its location to regulate various cellular processes such as endocytic recycling, secretion, and cytokinesis. However, our knowledge of its upstream regulators is still limited. Here, we identify the RAB-11-interacting protein-1 (REI-1) as a unique family of guanine nucleotide exchange factors (GEFs) for RAB-11 in Caenorhabditis elegans. Although REI-1 and its human homolog SH3-binding protein 5 do not contain any known Rab-GEF domains, they exhibited strong GEF activity toward Rab11 in vitro. In C. elegans, REI-1 is expressed in the germline and co-localizes with RAB-11 on the late-Golgi membranes. The loss of REI-1 specifically impaired the targeting of RAB-11 to the late-Golgi compartment and the recycling endosomes in embryos and further reduced the RAB-11 distribution to the cleavage furrow, which resulted in cytokinesis delay. These results suggest that REI-1 is a GEF specifically regulating the RAB-11 localization and functions in early embryos. Rab11 is a key regulator of post-Golgi membrane trafficking, but regulatory mechanisms affecting its activity are not well understood. Sakaguchi et al. identify C. elegans REI-1 and its mammalian homolog SH3BP5 as Rab11 guanine nucleotide exchange factors. In C. elegans, REI-1 is specifically required for RAB-11 regulation during embryonic cytokinesis.
AB - The small GTPase Rab11 dynamically changes its location to regulate various cellular processes such as endocytic recycling, secretion, and cytokinesis. However, our knowledge of its upstream regulators is still limited. Here, we identify the RAB-11-interacting protein-1 (REI-1) as a unique family of guanine nucleotide exchange factors (GEFs) for RAB-11 in Caenorhabditis elegans. Although REI-1 and its human homolog SH3-binding protein 5 do not contain any known Rab-GEF domains, they exhibited strong GEF activity toward Rab11 in vitro. In C. elegans, REI-1 is expressed in the germline and co-localizes with RAB-11 on the late-Golgi membranes. The loss of REI-1 specifically impaired the targeting of RAB-11 to the late-Golgi compartment and the recycling endosomes in embryos and further reduced the RAB-11 distribution to the cleavage furrow, which resulted in cytokinesis delay. These results suggest that REI-1 is a GEF specifically regulating the RAB-11 localization and functions in early embryos. Rab11 is a key regulator of post-Golgi membrane trafficking, but regulatory mechanisms affecting its activity are not well understood. Sakaguchi et al. identify C. elegans REI-1 and its mammalian homolog SH3BP5 as Rab11 guanine nucleotide exchange factors. In C. elegans, REI-1 is specifically required for RAB-11 regulation during embryonic cytokinesis.
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U2 - 10.1016/j.devcel.2015.09.013
DO - 10.1016/j.devcel.2015.09.013
M3 - Article
C2 - 26506309
AN - SCOPUS:84945117171
SN - 1534-5807
VL - 35
SP - 211
EP - 221
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -