Regulatory mechanism of the flavoprotein Tah18-dependent nitric oxide synthesis and cell death in yeast

Yuki Yoshikawa, Ryo Nasuno, Nobuhiro Kawahara, Akira Nishimura, Daisuke Watanabe, Hiroshi Takagi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Nitric oxide (NO) is a ubiquitous signaling molecule involved in the regulation of a large number of cellular functions. The regulatory mechanism of NO generation in unicellular eukaryotic yeast cells is poorly understood due to the lack of mammalian and bacterial NO synthase (NOS) orthologues, even though yeast produces NO under oxidative stress conditions. Recently, we reported that the flavoprotein Tah18, which was previously shown to transfer electrons to the iron-sulfur cluster protein Dre2, is involved in NOS-like activity in the yeast Saccharomyces cerevisiae. On the other hand, Tah18 was reported to promote apoptotic cell death after exposure to hydrogen peroxide (H2O2). Here, we showed that NOS-like activity requiring Tah18 induced cell death upon treatment with H2O2. Our experimental results also indicate that Tah18-dependent NO production and cell death are suppressed by enhancement of the interaction between Tah18 and its molecular partner Dre2. Our findings indicate that the Tah18-Dre2 complex regulates cell death as a molecular switch via Tah18-dependent NOS-like activity in response to environmental changes.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalNitric Oxide - Biology and Chemistry
Volume57
DOIs
Publication statusPublished - 2016 Jul 1
Externally publishedYes

Keywords

  • Cell death
  • Dre2
  • Nitric oxide
  • Nitric oxide synthase
  • Saccharomyces cerevisiae
  • Tah18
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research

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