TY - JOUR
T1 - Regulation of YB-1 gene expression by GATA transcription factors
AU - Yokoyama, Hisayuki
AU - Harigae, Hideo
AU - Takahashi, Shinichiro
AU - Takahashi, Satoru
AU - Furuyama, Kazumichi
AU - Kaku, Mitsuo
AU - Yamamoto, Masayuki
AU - Sasaki, Takeshi
N1 - Funding Information:
We thank Dr. N. Minegishi for providing GATA-1 and GATA-2 expression vectors and cDNAs. We also thank Dr. T. Miura and Ms. T. Saito for the excellent technical assistance and thank the staff in the Department of Rheumatology and Hematology for helpful discussions. This work is supported in part by Grant-in Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (to H.H., T.S.).
PY - 2003/3/28
Y1 - 2003/3/28
N2 - GATA-1 is a transcription factor essential for erythroid cell development, and knockdown of GATA-1 gene results in maturation arrest and transformation of erythroblasts. To clarify the mechanism that gives rise to this abnormal phenotype, genes that are aberrantly expressed in the spleen of heterozygous GATA-1 knockdown mutant mouse (referred to as GATA-1 mutant mouse) were identified by using cDNA array. One of these genes, YB-1, was found to be highly expressed in the spleen of GATA-1 mutant mouse. Reporter and electrophoretic mobility shift assays revealed that the proximal GATA element in 5′-UTR region of YB-1 gene functions positively in K562 cells. Furthermore, both GATA-1 and GATA-2, which were transiently expressed in COS-7 cells, bound to this element and activated the YB-1 promoter through this element. These results suggest that YB-1 functions under the regulation of GATA factors in erythroid differentiation and aberrant expression of YB-1 gene may result in dyserythropoiesis.
AB - GATA-1 is a transcription factor essential for erythroid cell development, and knockdown of GATA-1 gene results in maturation arrest and transformation of erythroblasts. To clarify the mechanism that gives rise to this abnormal phenotype, genes that are aberrantly expressed in the spleen of heterozygous GATA-1 knockdown mutant mouse (referred to as GATA-1 mutant mouse) were identified by using cDNA array. One of these genes, YB-1, was found to be highly expressed in the spleen of GATA-1 mutant mouse. Reporter and electrophoretic mobility shift assays revealed that the proximal GATA element in 5′-UTR region of YB-1 gene functions positively in K562 cells. Furthermore, both GATA-1 and GATA-2, which were transiently expressed in COS-7 cells, bound to this element and activated the YB-1 promoter through this element. These results suggest that YB-1 functions under the regulation of GATA factors in erythroid differentiation and aberrant expression of YB-1 gene may result in dyserythropoiesis.
KW - Erythropoiesis
KW - GATA factors
KW - YB-1 gene
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U2 - 10.1016/S0006-291X(03)00296-1
DO - 10.1016/S0006-291X(03)00296-1
M3 - Article
C2 - 12646178
AN - SCOPUS:0037470817
VL - 303
SP - 140
EP - 145
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -