Regulation of uncoupling protein 2 expression by long-chain fatty acids and hormones in bovine mammary epithelial cells.

Tomo Yonezawa, Masato Sanosaka, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    Although mammary epithelial cells are known to synthesize and accumulate triacylglycerol (TAG) in order to produce milk lipid in the cytosol, lipid and energy metabolism is still not fully understood. In this study, we assessed the effects of long-chain fatty acid (LCFA) on the accumulation of cytosolic TAG and uncoupling protein (UCP) 2 in cloned bovine mammary epithelial cells (bMEC). LCFAs significantly raised the expression of UCP2 mRNA and the accumulation of TAG. We observed the rapid elevation in UCP2 shown at 6h after LCFA treatment. Insulin (5-50 ng/ml) or dexamethasone (500 nM) significantly suppressed the expression of UCP2 mRNA. These results suggest that UCP2 play an important role of lipid and energy metabolism in mammary epithelial cells.

    Original languageEnglish
    Pages (from-to)280-285
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume375
    Issue number2
    DOIs
    Publication statusPublished - 2008 Oct 17

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Fingerprint Dive into the research topics of 'Regulation of uncoupling protein 2 expression by long-chain fatty acids and hormones in bovine mammary epithelial cells.'. Together they form a unique fingerprint.

    Cite this