TY - JOUR
T1 - Regulation of uncoupling protein 2 expression by long-chain fatty acids and hormones in bovine mammary epithelial cells.
AU - Yonezawa, Tomo
AU - Sanosaka, Masato
AU - Haga, Satoshi
AU - Kobayashi, Yosuke
AU - Katoh, Kazuo
AU - Obara, Yoshiaki
N1 - Funding Information:
This work was supported by a grant from Japan Society for Promotion of Science.
Funding Information:
The authors sincerely thank Dr. Komatsu T. (National Agricultural Research Organization, National Agricultural Center for Tohoku region, Iwate-ken, Japan) for the gift of bovine mammary gland samples. This work was supported by a grant from Japan Society for Promotion of Science.
Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2008/10/17
Y1 - 2008/10/17
N2 - Although mammary epithelial cells are known to synthesize and accumulate triacylglycerol (TAG) in order to produce milk lipid in the cytosol, lipid and energy metabolism is still not fully understood. In this study, we assessed the effects of long-chain fatty acid (LCFA) on the accumulation of cytosolic TAG and uncoupling protein (UCP) 2 in cloned bovine mammary epithelial cells (bMEC). LCFAs significantly raised the expression of UCP2 mRNA and the accumulation of TAG. We observed the rapid elevation in UCP2 shown at 6h after LCFA treatment. Insulin (5-50 ng/ml) or dexamethasone (500 nM) significantly suppressed the expression of UCP2 mRNA. These results suggest that UCP2 play an important role of lipid and energy metabolism in mammary epithelial cells.
AB - Although mammary epithelial cells are known to synthesize and accumulate triacylglycerol (TAG) in order to produce milk lipid in the cytosol, lipid and energy metabolism is still not fully understood. In this study, we assessed the effects of long-chain fatty acid (LCFA) on the accumulation of cytosolic TAG and uncoupling protein (UCP) 2 in cloned bovine mammary epithelial cells (bMEC). LCFAs significantly raised the expression of UCP2 mRNA and the accumulation of TAG. We observed the rapid elevation in UCP2 shown at 6h after LCFA treatment. Insulin (5-50 ng/ml) or dexamethasone (500 nM) significantly suppressed the expression of UCP2 mRNA. These results suggest that UCP2 play an important role of lipid and energy metabolism in mammary epithelial cells.
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U2 - 10.1016/j.bbrc.2008.08.021
DO - 10.1016/j.bbrc.2008.08.021
M3 - Article
C2 - 18706890
AN - SCOPUS:52049088501
VL - 375
SP - 280
EP - 285
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -