Regulation of transcription by dimerization of erythroid factor NF-E2 p45 with small Maf proteins

Kazuhiko Igarashi, Kohsuke Kataokat, Ken Itoh, Norio Hayashi, Makoto Nishizawa, Masayuki Yamamoto

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382 Citations (Scopus)

Abstract

TRANSCRIPTION factor NF-E2 is crucial for regulating erythroid-specific gene expression1. Cloning of the NF-E2 p45 protein has revealed that it contains a basic region-leucine zipper (b-zip) domain which associates with another unidentified protein (of relative molecular mass 18,000) to form functional NF-E2 (ref. 2). We show here that products of the maf proto-oncogene family3-5, MafF, MafG and MafK (the small Maf proteins) which possess a b-zip DNA-binding domain but lack a canonical transactivation domain 3, directly control the DNA-binding properties of p45 by heterodimeric association with p45. Whereas homodimers of the small Maf proteins act as negative regulators, heterodimers composed of Maf and p45 support active transcription in vivo. These results indicate that one (or all) of the small Maf proteins is the second constituent chain required for NF-E2 activity, and that negative as well as positive regulation can be achieved through an NF-E2 site, depending on the equilibrium concentrations of p45 and the Maf proteins inside erythroid cells.

Original languageEnglish
Pages (from-to)568-572
Number of pages5
JournalNature
Volume367
Issue number6463
DOIs
Publication statusPublished - 1994 Jan 1

ASJC Scopus subject areas

  • General

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