Regulation of the expression and activity of glucose and lactic acid metabolism-related genes by protein kinase c in skeletal muscle cells

Sho Otake, Masaki Kobayashi, Katsuya Narumi, Shotaro Sasaki, Yurika Kikutani, Ayako Furugen, Meguho Watanabe, Natsuko Takahashi, Jiro Ogura, Hiroaki Yamaguchi, Ken Iseki

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Protein kinase C (PKC) modulators are very attractive therapeutic targets in cancer. Since most cancer cells display increased glycolysis, elucidations of the effects of PKC activation on glycolysis is necessary for the development of effective medicine. In the present study, to clarify the role of PKC in the regulation of glycolysis, we examined the effect of phorbol 12-myristate 13-acetate (PMA), a PKC activator, on the expression and activity of glucose and lactic acid metabolism-related genes in human rhabdomyosarcoma cells (RD cells). In parallel to increases in glucose uptake and mRNA levels of glucose transporters (GLUTs) induced by PMA treatment for 6 h, the hexokinase (HK) mRNA level and activity were also significantly increased in RD cells. On the other hand, a significant increase in lactate dehydrogenase (LDH) mRNA level and activity was seen when the cells were incubated with PMA for 24 h, but not for 6 or 12 h, and was associated with lactic acid production. These effects by PMA treatment were markedly suppressed by Bisindolylmaleimide (BIM), a PKC inhibitor. Furthermore, chetomin, a hypoxia-inducible factor 1 (HIF-1) inhibitor, completely abrogated the increment of LDH mRNA level and activity as well as monocarboxylate transporter (MCT) 4, a lactic acid efflux transporter. In conclusion, we found that HK and LDH activity induced by PKC activation was associated with the glucose uptake and lactic acid level and that LDH and MCT4 are modulated by a common factor, HIF-1.

Original languageEnglish
Pages (from-to)1435-1439
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume36
Issue number9
DOIs
Publication statusPublished - 2013 Sep

Keywords

  • Glycolysis
  • Protein kinase C
  • Skeletal muscle

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint

Dive into the research topics of 'Regulation of the expression and activity of glucose and lactic acid metabolism-related genes by protein kinase c in skeletal muscle cells'. Together they form a unique fingerprint.

Cite this