Regulation of root greening by light and auxin/cytokinin signaling in Arabidopsis

Koichi Kobayashi, Shinsuke Baba, Takeshi Obayashi, Mayuko Sato, Kiminori Toyooka, Mika Keränen, Eva Mari Aro, Hidehiro Fukaki, Hiroyuki Ohta, Keiko Sugimoto, Tatsuru Masudaa

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

Tight coordination between plastid differentiation and plant development is best evidenced by the synchronized development of photosynthetic tissues and the biogenesis of chloroplasts. Here, we show that Arabidopsis thaliana roots demonstrate accelerated chlorophyll accumulation and chloroplast development when they are detached from shoots. However, this phenomenon is repressed by auxin treatment. Mutant analyses suggest that auxin transported from the shoot represses root greening via the function of INDOLE-3-ACETIC ACID14, AUXIN RESPONSE FACTOR7 (ARF7), and ARF19. Cytokinin signaling, on the contrary, is required for chlorophyll biosynthesis in roots. The regulation by auxin/cytokinin is dependent on the transcription factor LONG HYPOCOTYL5 (HY5), which is required for the expression of key chlorophyll biosynthesis genes in roots. The expression of yet another root greening transcription factor, GOLDEN2-LIKE2 (GLK2), was found to be regulated in opposing directions by auxin and cytokinin. Furthermore, both the hormone signaling and the GLK transcription factors modified the accumulation of HY5 in roots. Overexpression of GLKs in the hy5 mutant provided evidence that GLKs require HY5 to maximize their activities in root greening. We conclude that the combination of HY5 and GLKs, functioning downstream of light and auxin/cytokinin signaling pathways, is responsible for coordinated expression of the key genes in chloroplast biogenesis.

Original languageEnglish
Pages (from-to)1081-1095
Number of pages15
JournalPlant Cell
Volume24
Issue number3
DOIs
Publication statusPublished - 2012 Mar

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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