Regulation of podocalyxin trafficking by Rab small GTPases in epithelial cells

Paulina S. Mrozowska, Mitsunori Fukuda

    Research output: Contribution to journalComment/debatepeer-review

    4 Citations (Scopus)

    Abstract

    The characteristic feature of polarity establishment in MDCK II cells is transcytosis of apical glycoprotein podocalyxin (PCX) from the outer plasma membrane to the newly formed apical domain. This transcytotic event consists of multiple steps, including internalization from the plasma membrane, transport through early endosomes and Rab11-positive recycling endosomes, and delivery to the apical membrane. These steps are known to be tightly coordinated by Rab small GTPases, which act as molecular switches cycling between active GTP-bound and inactive GDP-bound states. However, our knowledge regarding which sets of Rabs regulate particular steps of PCX trafficking was rather limited. Recently, we have performed a comprehensive analysis of Rab GTPase engagement in the transcytotic pathway of PCX during polarity establishment in 2-dimensional (2D) and 3-dimensional (3D) MDCK II cell cultures. In this Commentary we summarize our findings and set them in the context of previous reports.

    Original languageEnglish
    Pages (from-to)231-238
    Number of pages8
    JournalSmall GTPases
    Volume7
    Issue number4
    DOIs
    Publication statusPublished - 2016 Oct 1

    Keywords

    • MDCK cells
    • Rab small GTPases
    • epithelial cell polarity
    • podocalyxin/gp135
    • recycling endosome
    • transcytosis

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology

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