TY - JOUR
T1 - Regulation of plasma glycero-lysophospholipid levels by lipoprotein metabolism
AU - Kurano, Makoto
AU - Kano, Kuniyuki
AU - Hara, Masumi
AU - Tsukamoto, Kazuhisa
AU - Aoki, Junken
AU - Yatomi, Yutaka
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number 16H06236 (M.K.), Japan Heart Foundation and Astellas Grant for Research on Atherosclerosis Update (M.K.), and MSD Life Science Foundation, Public Interest Incorporated Foundation (M.K.), Leading Advanced Projects for medical innovation (LEAP) from AMED (J.A. and Y.Y.), and a Grant-in-Aid for Scientific Research on Innovative Areas 15H05906 (Y.Y.).
Funding Information:
This work was supported by JSPS KAKENHI Grant Number 16H06236 (M.K.), Japan Heart Foundation and Astellas Grant for Research on Atherosclerosis Update (M.K.), and MSD Life Science Foundation, Public Interest Incorporated Foundation (M.K.), Leading Advanced Projects for medical innovation (LEAP) from AMED ( J.A. and Y.Y.), and a Grant-in-Aid for Scientific Research on Innovative Areas 15H05906 (Y.Y.).
PY - 2019
Y1 - 2019
N2 - Glycero-lysophospholipids, such as lysophosphatidic acids and lysophosphatidylserine, are gathering attention, since specific receptors have been identified. Most of these compounds have been proposed to be bound to albumin, while their associations with lipoproteins have not been fully elucidated. Therefore, in this study, we aimed to investigate the contents of glycero-lysophospholipids (lysophosphatidic acids, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, and lysophosphatidylserine) on lipoproteins and the modulation of their metabolism by lipoprotein metabolism. We observed that moderate amounts of glycero-lysophospholipids, with the exception of lysophosphatidylserine, were distributed on the LDL and HDL fractions, and glycero-lysophospholipids that had bound to albumin were observed in lipoprotein fractions when they were co-incubated. The overexpression of cholesteryl ester transfer protein decreased the plasma levels of lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol and it increased their contents in apoB-containing lipoproteins, while it decreased their contents in HDL and lipoprotein-depleted fractions in mice. The overexpression of the LDL receptor (LDLr) decreased the plasma levels of lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol and decreased the contents of these compounds in the LDL, HDL, and lipoprotein-depleted fractions, while the knockdown of the LDLr increased them. These results suggest the potential importance of glycero-lysophospholipids in the pleiotropic effects of lipoproteins as well as the importance of lipoprotein metabolism in the regulation of glycero-lysophospholipids.
AB - Glycero-lysophospholipids, such as lysophosphatidic acids and lysophosphatidylserine, are gathering attention, since specific receptors have been identified. Most of these compounds have been proposed to be bound to albumin, while their associations with lipoproteins have not been fully elucidated. Therefore, in this study, we aimed to investigate the contents of glycero-lysophospholipids (lysophosphatidic acids, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, and lysophosphatidylserine) on lipoproteins and the modulation of their metabolism by lipoprotein metabolism. We observed that moderate amounts of glycero-lysophospholipids, with the exception of lysophosphatidylserine, were distributed on the LDL and HDL fractions, and glycero-lysophospholipids that had bound to albumin were observed in lipoprotein fractions when they were co-incubated. The overexpression of cholesteryl ester transfer protein decreased the plasma levels of lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol and it increased their contents in apoB-containing lipoproteins, while it decreased their contents in HDL and lipoprotein-depleted fractions in mice. The overexpression of the LDL receptor (LDLr) decreased the plasma levels of lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol, and lysophosphatidylinositol and decreased the contents of these compounds in the LDL, HDL, and lipoprotein-depleted fractions, while the knockdown of the LDLr increased them. These results suggest the potential importance of glycero-lysophospholipids in the pleiotropic effects of lipoproteins as well as the importance of lipoprotein metabolism in the regulation of glycero-lysophospholipids.
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U2 - 10.1042/BCJ20190498
DO - 10.1042/BCJ20190498
M3 - Article
C2 - 31746967
AN - SCOPUS:85076097373
VL - 476
SP - 3565
EP - 3581
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 23
ER -