Regulation of PLAP-1 expression in periodontal ligament cells

S. Yamada, Y. Ozawa, M. Tomoeda, R. Matoba, K. Matsubara, S. Murakami

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36 Citations (Scopus)


Periodontal-ligament-associated protein-1 (PLAP-1) is preferentially expressed in the periodontal ligament (PDL) and encodes a novel small leucine-rich repeat proteoglycan protein. PLAP-1 expression was induced during the course of cytodifferentiation of PDL cells into mineralized-tissue-forming cells in vitro, suggesting the possible involvement of PLAP-1 in the mineralization process of PDL cells. In this study, we hypothesized that PLAP-1 expression is regulated by mineralization-related cytokines in PDL cells. PLAP-1 expression was clearly down-regulated when the cytodifferentiation of PDL cells was reversibly inhibited by fibroblast growth factor-2 (FGF-2). In contrast, bone morphogenetic protein-2 (BMP-2) enhanced PLAP-1 expression. Up-regulation of PLAP-1 expression by BMP-2 was confirmed at the protein level when PDL cells were immunostained with anti-PLAP-1 polyclonal antibody. These results revealed the cytokine-mediated regulatory mechanisms of PLAP-1 expression and suggested that PLAP-1 expression may be associated with the process of cytodifferentiation of PDL cells.

Original languageEnglish
Pages (from-to)447-451
Number of pages5
JournalJournal of dental research
Issue number5
Publication statusPublished - 2006 May
Externally publishedYes


  • BMP-2
  • FGF-2
  • Mineralization
  • PLAP-1
  • Periodontal ligament cells

ASJC Scopus subject areas

  • Dentistry(all)


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