Regulation of Output Current of L-Lactate Sensors Based on Alternate Deposition of Avidin and Biotinylated Lactate Oxidase on Electrode Surface Through Avidin/Biotin Complexation

Jun ichi Anzai, Hiroki Takeshita, Tomonori Hoshi, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

An alternate and repeated deposition of avidin and biotinylated lactate oxidase (LOx) gave a thin film on the surface of platinum electrode. Amperometric response of L-lactate sensors thus prepared was enhanced stepwise by increasing the number of LOx layers. The enhanced response contributed to the extension of the lower detection limit of the sensor. The response time of the sensors was satisfactorily fast (ca. 10 s), irrespective of the number of LOx layers.

Original languageEnglish
Pages (from-to)520-522
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume43
Issue number3
DOIs
Publication statusPublished - 1995

Keywords

  • L-lactate
  • avidin/biotin system
  • lactate oxidase
  • protein multilayer
  • sensor

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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