Regulation of neural stem cell differentiation and proliferation by self-organized honeycomb-patterned films

Akinori Tsuruma; Masaru Tanaka; Nobuyuki Fukushima; Sadaaki Yamamoto; Masatsugu Shimomura

Regulation of neural stem cell differentiation and proliferation by self-organized honeycomb-patterned films

Akinori Tsuruma, Masaru Tanaka, Nobuyuki Fukushima, Sadaaki Yamamoto, Masatsugu Shimomura

Research output: Contribution to conference › Paper › peer-review

Abstract

We have succeeded in preparing porous biodegradable polymer films with highly regular aligned pores in the shape of honeycomb (honeycomb film) by a simple casting method. In this report, we cultured NSCs on honeycomb films (pore size, 3, 5, 8, and 10 μm) and investigated influences of the pore size on differentiation and proliferation of NSCs. On honeycomb films with pore size of > 5 μm, NSCs differentiated into neurons. The neurons extended neurites along the honeycomb patterns, and neurite morphological changes depended on pore size of honeycomb films. On the other hands, NSCs proliferated and formed spheroids on the honeycomb films with pore size of 3 μm. This study shows that the honeycomb films are potential scaffolds for neural tissue engineering.

Original language	English
Pages	1911
Number of pages	1
Publication status	Published - 2006
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26

Other

Other	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	06/5/24 → 06/5/26

Keywords

Differentiation
Neural network
Neural stem cell
Neuron
Proliferation
Tissue engineering

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Regulation of neural stem cell differentiation and proliferation by self-organized honeycomb-patterned films",

abstract = "We have succeeded in preparing porous biodegradable polymer films with highly regular aligned pores in the shape of honeycomb (honeycomb film) by a simple casting method. In this report, we cultured NSCs on honeycomb films (pore size, 3, 5, 8, and 10 μm) and investigated influences of the pore size on differentiation and proliferation of NSCs. On honeycomb films with pore size of > 5 μm, NSCs differentiated into neurons. The neurons extended neurites along the honeycomb patterns, and neurite morphological changes depended on pore size of honeycomb films. On the other hands, NSCs proliferated and formed spheroids on the honeycomb films with pore size of 3 μm. This study shows that the honeycomb films are potential scaffolds for neural tissue engineering.",

keywords = "Differentiation, Neural network, Neural stem cell, Neuron, Proliferation, Tissue engineering",

author = "Akinori Tsuruma and Masaru Tanaka and Nobuyuki Fukushima and Sadaaki Yamamoto and Masatsugu Shimomura",

year = "2006",

language = "English",

pages = "1911",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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TY - CONF

T1 - Regulation of neural stem cell differentiation and proliferation by self-organized honeycomb-patterned films

AU - Tsuruma, Akinori

AU - Tanaka, Masaru

AU - Fukushima, Nobuyuki

AU - Yamamoto, Sadaaki

AU - Shimomura, Masatsugu

PY - 2006

Y1 - 2006

N2 - We have succeeded in preparing porous biodegradable polymer films with highly regular aligned pores in the shape of honeycomb (honeycomb film) by a simple casting method. In this report, we cultured NSCs on honeycomb films (pore size, 3, 5, 8, and 10 μm) and investigated influences of the pore size on differentiation and proliferation of NSCs. On honeycomb films with pore size of > 5 μm, NSCs differentiated into neurons. The neurons extended neurites along the honeycomb patterns, and neurite morphological changes depended on pore size of honeycomb films. On the other hands, NSCs proliferated and formed spheroids on the honeycomb films with pore size of 3 μm. This study shows that the honeycomb films are potential scaffolds for neural tissue engineering.

AB - We have succeeded in preparing porous biodegradable polymer films with highly regular aligned pores in the shape of honeycomb (honeycomb film) by a simple casting method. In this report, we cultured NSCs on honeycomb films (pore size, 3, 5, 8, and 10 μm) and investigated influences of the pore size on differentiation and proliferation of NSCs. On honeycomb films with pore size of > 5 μm, NSCs differentiated into neurons. The neurons extended neurites along the honeycomb patterns, and neurite morphological changes depended on pore size of honeycomb films. On the other hands, NSCs proliferated and formed spheroids on the honeycomb films with pore size of 3 μm. This study shows that the honeycomb films are potential scaffolds for neural tissue engineering.

KW - Differentiation

KW - Neural network

KW - Neural stem cell

KW - Neuron

KW - Proliferation

KW - Tissue engineering

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M3 - Paper

AN - SCOPUS:33749848965

SP - 1911

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Regulation of neural stem cell differentiation and proliferation by self-organized honeycomb-patterned films

Abstract

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Keywords

ASJC Scopus subject areas

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