TY - JOUR
T1 - Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells
AU - Yonezawa, Tomo
AU - Haga, Satoshi
AU - Kobayashi, Yosuke
AU - Katoh, Kazuo
AU - Obara, Yoshiaki
N1 - Funding Information:
This work was supported by a grant from Japan Society for Promotion of Science.
PY - 2008/11/7
Y1 - 2008/11/7
N2 - Hormone-sensitive lipase was firstly identified as an epinephrine-induced lipase in adipocyte. HSL mRNA was detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and bovine lactating mammary gland. Saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Treatment with insulin (5-10 ng/ml), dexamethasone (50-250 nM) or GH (50 ng/ml) induced down-regulation of HSL. These results suggest that HSL was regulated by fatty acids and some hormones in mammary epithelial cells and thereby play an important role of lipid and energy metabolism.
AB - Hormone-sensitive lipase was firstly identified as an epinephrine-induced lipase in adipocyte. HSL mRNA was detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and bovine lactating mammary gland. Saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Treatment with insulin (5-10 ng/ml), dexamethasone (50-250 nM) or GH (50 ng/ml) induced down-regulation of HSL. These results suggest that HSL was regulated by fatty acids and some hormones in mammary epithelial cells and thereby play an important role of lipid and energy metabolism.
KW - Dexamethasone
KW - Hormone-sensitive lipase
KW - Insulin
KW - Mammary epithelial cell
KW - Saturated fatty acids
UR - http://www.scopus.com/inward/record.url?scp=52049112973&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52049112973&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.08.075
DO - 10.1016/j.bbrc.2008.08.075
M3 - Article
C2 - 18755148
AN - SCOPUS:52049112973
VL - 376
SP - 36
EP - 39
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -