Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells

Tomo Yonezawa, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Hormone-sensitive lipase was firstly identified as an epinephrine-induced lipase in adipocyte. HSL mRNA was detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and bovine lactating mammary gland. Saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Treatment with insulin (5-10 ng/ml), dexamethasone (50-250 nM) or GH (50 ng/ml) induced down-regulation of HSL. These results suggest that HSL was regulated by fatty acids and some hormones in mammary epithelial cells and thereby play an important role of lipid and energy metabolism.

    Original languageEnglish
    Pages (from-to)36-39
    Number of pages4
    JournalBiochemical and biophysical research communications
    Volume376
    Issue number1
    DOIs
    Publication statusPublished - 2008 Nov 7

    Keywords

    • Dexamethasone
    • Hormone-sensitive lipase
    • Insulin
    • Mammary epithelial cell
    • Saturated fatty acids

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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