Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells

Tomo Yonezawa; Satoshi Haga; Yosuke Kobayashi; Kazuo Katoh; Yoshiaki Obara

doi:10.1016/j.bbrc.2008.08.075

Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells

Tomo Yonezawa, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Hormone-sensitive lipase was firstly identified as an epinephrine-induced lipase in adipocyte. HSL mRNA was detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and bovine lactating mammary gland. Saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of HSL mRNA in a time- and concentration-dependent manner in bMEC. Treatment with insulin (5-10 ng/ml), dexamethasone (50-250 nM) or GH (50 ng/ml) induced down-regulation of HSL. These results suggest that HSL was regulated by fatty acids and some hormones in mammary epithelial cells and thereby play an important role of lipid and energy metabolism.

Original language	English
Pages (from-to)	36-39
Number of pages	4
Journal	Biochemical and biophysical research communications
Volume	376
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.08.075
Publication status	Published - 2008 Nov 7

Keywords

Dexamethasone
Hormone-sensitive lipase
Insulin
Mammary epithelial cell
Saturated fatty acids

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Regulation of hormone-sensitive lipase expression by saturated fatty acids and hormones in bovine mammary epithelial cells. / Yonezawa, Tomo; Haga, Satoshi; Kobayashi, Yosuke; Katoh, Kazuo; Obara, Yoshiaki.

In: Biochemical and biophysical research communications, Vol. 376, No. 1, 07.11.2008, p. 36-39.

Research output: Contribution to journal › Article › peer-review

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