Regulation of hormone metabolism in Arabidopsis seeds: Phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism

Mitsunori Seo, Atsushi Hanada, Ayuko Kuwahara, Akira Endo, Masanori Okamoto, Yukika Yamauchi, Helen North, Annie Marion-Poll, Tai Ping Sun, Tomokazu Koshiba, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara

    Research output: Contribution to journalArticlepeer-review

    278 Citations (Scopus)

    Abstract

    In a wide range of plant species, seed germination is regulated antagonistically by two plant hormones, abscisic acid (ABA) and gibberellin (GA). In the present study, we have revealed that ABA metabolism (both biosynthesis and inactivation) was phytochrome-regulated in an opposite fashion to GA metabolism during photoreversible seed germination in Arabidopsis. Endogenous ABA levels were decreased by irradiation with a red (R) light pulse in dark-imbibed seeds pre-treated with a far-red (FR) light pulse, and the reduction in ABA levels in response to R light was inhibited in a phytochrome B (PHYB)-deficient mutant. Expression of an ABA biosynthesis gene, AtNCED6, and the inactivation gene, CYP707A2, was regulated in a photoreversible manner, suggesting a key role for the genes in PHYB-mediated regulation of ABA metabolism. Abscisic acid-deficient mutants such as nced6-1, aba2-2 and aao3-4 exhibited an enhanced ability to germinate relative to wild type when imbibed in the dark after irradiation with an FR light pulse. In addition, the ability to synthesize GA was improved in the aba2-2 mutant compared with wild type during dark-imbibition after an FR light pulse. Activation of GA biosynthesis in the aba2-2 mutant was also observed during seed development. These data indicate that ABA is involved in the suppression of GA biosynthesis in both imbibed and developing seeds. Spatial expression patterns of the AtABA2 and AAO3 genes, responsible for last two steps of ABA biosynthesis, were distinct from that of the GA biosynthesis gene, AtGA3ox2, in both imbibed and developing seeds, suggesting that biosynthesis of ABA and GA in seeds occurs in different cell types.

    Original languageEnglish
    Pages (from-to)354-366
    Number of pages13
    JournalPlant Journal
    Volume48
    Issue number3
    DOIs
    Publication statusPublished - 2006 Nov

    Keywords

    • Abscisic acid
    • Gibberellin
    • Hormone metabolism
    • Phytochrome
    • Seed development
    • Seed germination

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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