TY - JOUR
T1 - Regulation of expression of the steroidogenic acute regulatory (StAR) protein by ACTH in bovine adrenal fasciculata cells
AU - Nishikawa, Tetsuo
AU - Sasano, Hironobu
AU - Omura, Masao
AU - Suematsu, Sachiko
N1 - Funding Information:
We are very grateful to Prof. Douglas M. Stocco, Department of Cell Biology & Biochemistry, School of Medicine, Texas Tech University, Health Science Center for his kind supply of antiserum for the StAR protein. This work was supported in part by a Grant from the Ministry of Health and Welfare, “Disorders of Adrenal Hormone Research” Committee, Japan, and in part by a Grant from the Labor Welfare Corporation, Tokyo.
PY - 1996/6/5
Y1 - 1996/6/5
N2 - Immunocytochemical studies and immunoblotting analysis demonstrated that there exists the StAR protein in bovine adrenal fasciculata cells, and ACTH activated expression of the StAR protein. Then roles of intracellular signal transduction systems in the regulation of expression of the StAR protein were studied. The addition of Bt2cAMP and forskolin, or phorbol ester plus calcium ionophore 23187 activated expression of the StAR protein as well as cortisol production, suggesting that cyclic AMP- or protein kinase C-dependent process plays a crucial role in the regulation of expression of the StAR protein. Activating effects of ACTH which activates cyclic AMP formation on the StAR protein and cortisol production were inhibited by pretreatment with calphostin C which is a protein kinase C inhibitor, suggesting that ACTH enhances expression of the StAR protein possibly via both of two signal transduction systems such as cyclic AMP- and protein kinase C-dependent processes.
AB - Immunocytochemical studies and immunoblotting analysis demonstrated that there exists the StAR protein in bovine adrenal fasciculata cells, and ACTH activated expression of the StAR protein. Then roles of intracellular signal transduction systems in the regulation of expression of the StAR protein were studied. The addition of Bt2cAMP and forskolin, or phorbol ester plus calcium ionophore 23187 activated expression of the StAR protein as well as cortisol production, suggesting that cyclic AMP- or protein kinase C-dependent process plays a crucial role in the regulation of expression of the StAR protein. Activating effects of ACTH which activates cyclic AMP formation on the StAR protein and cortisol production were inhibited by pretreatment with calphostin C which is a protein kinase C inhibitor, suggesting that ACTH enhances expression of the StAR protein possibly via both of two signal transduction systems such as cyclic AMP- and protein kinase C-dependent processes.
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U2 - 10.1006/bbrc.1996.0838
DO - 10.1006/bbrc.1996.0838
M3 - Article
C2 - 8660356
AN - SCOPUS:0029945174
VL - 223
SP - 12
EP - 18
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -