Regulation of cytotoxic T lymphocyte triggering by PIR-B on dendritic cells

Shota Endo, Yuzuru Sakamoto, Eiji Kobayashi, Akira Nakamura, Toshiyuki Takai

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Priming of cytotoxic T lymphocytes (CTLs) by dendritic cells (DCs) is crucial for elimination of pathogens and malignant cells. To activate CTLs, DCs present antigenic peptide-complexed MHC class I molecules (MHC-I) that will be recognized by the CTLs with T cell receptors and CD8 molecules. Here we show that paired Ig-like receptor (PIR)-B, an MHC-I receptor expressed on antigen-presenting cells, can regulate CTL triggering by blocking the access of CD8 molecules to MHC-I. PIR-B-deficient DCs evoked CTLs more efficiently, leading to accelerated graft and tumor rejection. PIR-B+ non-DC transfectant cells served as less efficient stimulators and targets for CTLs than PIR-B- cells at the effector phase in vitro. On surface plasmon resonance analysis, PIR-B and CD8αα were revealed to compete in binding to MHC-I. Our results may provide a novel strategy for regulating CTL-mediated immunity and diseases in a sterical manner.

Original languageEnglish
Pages (from-to)14515-14520
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number38
DOIs
Publication statusPublished - 2008 Sep 23

Keywords

  • CTL priming
  • MHC class I
  • Regulatory receptor

ASJC Scopus subject areas

  • General

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