TY - JOUR
T1 - Regulation of Cell Volume by Nanosecond Pulsed Electric Fields
AU - Yang, Qi
AU - Kajimoto, Shinji
AU - Kobayashi, Yuki
AU - Hiramatsu, Hirotsugu
AU - Nakabayashi, Takakazu
N1 - Funding Information:
This work was financially supported by JSPS KAKENHI Grants JP17H05869 (T.N.), JP19H02666 (S.K.), and JP20H04689 (S.K.) from the Ministry of Education, Culture, Sports, Science and Technology in Japan.
Publisher Copyright:
© 2021 American Chemical Society
PY - 2021/9/30
Y1 - 2021/9/30
N2 - Stimulation of cells by nanosecond pulsed electric fields (nsPEFs) has attracted attention as a technology for medical applications such as cancer treatment. nsPEFs have been shown to affect intracellular environments without significant damage to cell membranes; however, the mechanism underlying the effect of nsPEFs on cells remains unclear. In this study, we constructed electrodes for applying nsPEFs and analyzed the change in volume of a single cell due to nsPEFs using fluorescence and Raman microscopy. It was shown that the direction of the change depended on the applied electric field; expansion due to the influx of water was observed at high electric field, and cell shrinkage was observed at low electric field. The change in cell volume was correlated to the change in the intracellular Ca2+concentration, and nsPEFs-induced shrinking was not observed when the Ca2+-free medium was used. This result suggests that the cell shrinkage is related to the regulatory volume decrease where the cell adjusts the increase in intracellular Ca2+concentration, inducing the efflux of ions and water from the cell.
AB - Stimulation of cells by nanosecond pulsed electric fields (nsPEFs) has attracted attention as a technology for medical applications such as cancer treatment. nsPEFs have been shown to affect intracellular environments without significant damage to cell membranes; however, the mechanism underlying the effect of nsPEFs on cells remains unclear. In this study, we constructed electrodes for applying nsPEFs and analyzed the change in volume of a single cell due to nsPEFs using fluorescence and Raman microscopy. It was shown that the direction of the change depended on the applied electric field; expansion due to the influx of water was observed at high electric field, and cell shrinkage was observed at low electric field. The change in cell volume was correlated to the change in the intracellular Ca2+concentration, and nsPEFs-induced shrinking was not observed when the Ca2+-free medium was used. This result suggests that the cell shrinkage is related to the regulatory volume decrease where the cell adjusts the increase in intracellular Ca2+concentration, inducing the efflux of ions and water from the cell.
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U2 - 10.1021/acs.jpcb.1c06058
DO - 10.1021/acs.jpcb.1c06058
M3 - Article
C2 - 34519209
AN - SCOPUS:85115978172
VL - 125
SP - 10692
EP - 10700
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
IS - 38
ER -