Regulation of B-Z conformational transition and complex formation with a z-form binding protein by introduction of constraint to double-stranded DNA by using a DNA nanoscaffold

Masayuki Endo, Masahiro Inoue, Yuki Suzuki, Chigusa Masui, Hironobu Morinaga, Kumi Hidaka, Hiroshi Sugiyama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

B-Z transition: A constrained and rotatable double-stranded DNA, in which the rotational freedom was controlled by its placement into a DNA nanoscaffold, was designed (see scheme). The Z α β protein bound preferentially to the rotatable strand rather than the constrained strand, even using the same CG sequence.

Original languageEnglish
Pages (from-to)16887-16890
Number of pages4
JournalChemistry - A European Journal
Volume19
Issue number50
DOIs
Publication statusPublished - 2013 Dec 9
Externally publishedYes

Keywords

  • DNA
  • atomic force microscopy
  • proteins
  • single-molecule studies

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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