TY - JOUR
T1 - Regulation of adipocyte differentiation of bone marrow stromal cells by transcription factor GATA-2
AU - Okitsu, Yoko
AU - Takahashi, Shinichiro
AU - Minegishi, Naoko
AU - Kameoka, Junichi
AU - Kaku, Mitsuo
AU - Yamamoto, Masayuki
AU - Sasaki, Takeshi
AU - Harigae, Hideo
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, and Culture of Japan (to H.H. and T.S.).
PY - 2007/12/14
Y1 - 2007/12/14
N2 - GATA-2 is a transcription factor expressed in stem and progenitor cells of various cell lineages. In this study, we examined the role of GATA-2 in the differentiation of adipocytes, which are the main components of the microenvironment for hematopoiesis. Suppression of GATA-2 in TBR343, a preadipocytic stromal cell line, resulted in the acceleration of adipocyte differentiation. Conversely, overexpression of GATA-2 induced the resistance of adipocyte differentiation. In addition, regulatory elements of the GATA-2 gene in TBR343 are the same as those in hematopoietic cells. These results suggest that GATA-2 maintains a hematopoietic microenvironment by regulating the differentiation of adipocytes.
AB - GATA-2 is a transcription factor expressed in stem and progenitor cells of various cell lineages. In this study, we examined the role of GATA-2 in the differentiation of adipocytes, which are the main components of the microenvironment for hematopoiesis. Suppression of GATA-2 in TBR343, a preadipocytic stromal cell line, resulted in the acceleration of adipocyte differentiation. Conversely, overexpression of GATA-2 induced the resistance of adipocyte differentiation. In addition, regulatory elements of the GATA-2 gene in TBR343 are the same as those in hematopoietic cells. These results suggest that GATA-2 maintains a hematopoietic microenvironment by regulating the differentiation of adipocytes.
KW - Adipocyte differentiation
KW - Bone marrow
KW - GATA-2
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U2 - 10.1016/j.bbrc.2007.10.031
DO - 10.1016/j.bbrc.2007.10.031
M3 - Article
C2 - 17950250
AN - SCOPUS:35448950375
VL - 364
SP - 383
EP - 387
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -