Regulation by estrogen and progesterone of protein kinases in rabbit myometrium

Kazuo Matsui, Kenji Higashi, Kohji Miyazaki, Masao Maeyama, Kohji Fukunaga, Eishichi Miyamoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We report on hormonal regulation of protein kinases in the rabbit myometrium. The injection of estrogen or progesterone produced 5.2�0.7- or 4.7�0.4-fold increases, respectively, of the total activity when histone was used as the substrate in the cytosol fraction of the tissue. The analysis of the activity by DEAE-cellulose column chromatography revealed that estrogen had no effect on increases in the levels in both types I and II cAMP-dependent protein kinases, on the basis of mg protein, while progesterone produced a 50% decrease in type I cAM P-dependent protein kinase and a 50% increase in type l! cAMP dependent protein kinase. Thus, the activity ratio of types I! to type I did not change by treatment with estrogen and increased from 2.8 to 7.9 with progesterone administration. As the activity patterns by treatment with human chorionic gonadotropin closely resembled those seen with progesterone, our findings in case of the latter hormone are probably valid. The results indicate that the levels of protein kinases in the myometrium are altered in response to estrogen and progesterone and may possibly be involved in the physiological functions of the tissue.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalThe Japanese Journal of Pharmacology
Volume33
Issue number2
DOIs
Publication statusPublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology

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