Regulating cellular behavior on few-layer reduced graphene oxide films with well-controlled reduction states

Xuetao Shi, Haixin Chang, Song Chen, Chen Lai, Ali Khademhosseini, Hongkai Wu

    Research output: Contribution to journalArticlepeer-review

    153 Citations (Scopus)

    Abstract

    Understanding the effect of graphene on cellular behavior is important for enabling a range of new biological and biomedical applications. However, due to the complexity of cell responses and graphene surface states, regulating cellular behaviors on graphene or its derivatives is still a great challenge. To address this challenge we have developed a novel, facile route to regulate the cellular behaviors on few-layer reduced graphene oxide (FRGO) films by controlling the reduction states of graphene oxide. Our results indicate that the surface oxygen content of FRGO has a strong influence on cellular behavior, with the best performance for cell attachment, proliferation and phenotype being obtained in moderately reduced FRGO. Cell performance decreased significantly as the FRGO was highly reduced. Moderate performance was found in non-reduced pure graphene oxide and control glass slides. Our results highlight the important role of surface physicochemical characteristics of graphene and its derivatives in their interactions with biocomponents, and may have great potential in enabling the utility of graphene based materials in various biomedical and bioelectronic applications. Regulating cellular behaviors on few-layer reduced graphene oxide (FRGO) films are explored by delicately controlling the reduction states of graphene oxide. Importantly, the results indicate that the surface oxygenous content of FRGO has a strong influence on cellular behaviors, and therefore can be tuned in a facile way to regulate cellular behaviors on FRGO.

    Original languageEnglish
    Pages (from-to)751-759
    Number of pages9
    JournalAdvanced Functional Materials
    Volume22
    Issue number4
    DOIs
    Publication statusPublished - 2012 Feb 22

    Keywords

    • cellular behavior
    • controlled reduction
    • few-layer graphene oxide
    • regulate

    ASJC Scopus subject areas

    • Chemistry(all)
    • Materials Science(all)
    • Condensed Matter Physics

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