Regional variation of excitatory and inhibitory amino acid-induced responses in rat dissociated CNS neurons

Satoru Ebihara, Tamotsu Takishima, Tetsuya Shirasaki, Norio Akaike

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    8 Citations (Scopus)

    Abstract

    Regional differences in glutamate (Glu), aspartate (Asp), γ-aminobutyric acid (GABA) and glycine (Gly) responses in CNS neurons were investigated by means of the whole-cell mode of the patch-clamp technique. The neurons were freshly dissociated from rat cortex, limbic system (hippocampal CA1 region), diencephalon (ventromedial hypothalamus), medulla (nucleus paragigantocellularis lateralis) and spinal cord (spinal dorsal horn). The current amplitudes induced by Glu and GABA did not show any regional differences whereas those of Asp- and Gly-induced responses were significantly different among CNS regions. The enhancement of Asp response by Gly was observed in all regions, and the facilitatory ratio did not differ among these regions. Even though the NMDA response in cortical neurons was significantly greater than that in spinal neurons, the ratios of NMDA response facilitation by Gly were also the same in both regions. When the current amplitudes induced by individual amino acids were estimated for the unit surface area of respective neurons (current density), the Glu, Asp and Gly responses showed regional heterogeneity whereas the GABA response did not.

    Original languageEnglish
    Pages (from-to)61-71
    Number of pages11
    JournalNeuroscience Research
    Volume14
    Issue number1
    DOIs
    Publication statusPublished - 1992 Jun

    Keywords

    • Aspartate
    • CNS neurons
    • GABA
    • Glutamate
    • Glycine
    • Whole-cell voltage-clamp

    ASJC Scopus subject areas

    • Neuroscience(all)

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