Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain

Kazuhiro Takahashi; Kazuhito Totsune; Masahiko Sone; Osamu Murakami; Fumitoshi Satoh; Zenei Arihara; Hironobu Sasano; Kazumi Iino; Toraichi Mouri

doi:10.1016/S0196-9781(98)00012-6

Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain

Kazuhiro Takahashi, Kazuhito Totsune, Masahiko Sone, Osamu Murakami, Fumitoshi Satoh, Zenei Arihara, Hironobu Sasano, Kazumi Iino, Toraichi Mouri

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT- PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pons, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pons. Reverse phase high- performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain.

Original language	English
Pages (from-to)	643-647
Number of pages	5
Journal	Peptides
Volume	19
Issue number	4
DOIs	https://doi.org/10.1016/S0196-9781(98)00012-6
Publication status	Published - 1998 Apr

Keywords

Human brain
PCR
RT-PCR
Radioimmunoassay
Urocortin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/S0196-9781(98)00012-6

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Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain. / Takahashi, Kazuhiro; Totsune, Kazuhito; Sone, Masahiko; Murakami, Osamu; Satoh, Fumitoshi; Arihara, Zenei; Sasano, Hironobu; Iino, Kazumi; Mouri, Toraichi.

In: Peptides, Vol. 19, No. 4, 04.1998, p. 643-647.

Research output: Contribution to journal › Article › peer-review

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title = "Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain",

abstract = "Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT- PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pons, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pons. Reverse phase high- performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain.",

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author = "Kazuhiro Takahashi and Kazuhito Totsune and Masahiko Sone and Osamu Murakami and Fumitoshi Satoh and Zenei Arihara and Hironobu Sasano and Kazumi Iino and Toraichi Mouri",

note = "Funding Information: We are grateful to K. Kikuchi for her excellent technical assistance. This study has been supported partly by a research grant for medical science from the Nippon University Alumni Association (to M. S.), a Grant-in-aid for Scientific Research (C; No. 09670117) from the Ministry of Education, Science, Sports and Culture of Japan (to K. T.), a Grant-in-aid for the Brain Science Research from the Ministry of Health and Welfare of Japan (to K. T.), and by the Gonryou Medical Foundation (to K. T.).",

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AU - Takahashi, Kazuhiro

AU - Totsune, Kazuhito

AU - Sone, Masahiko

AU - Murakami, Osamu

AU - Satoh, Fumitoshi

AU - Arihara, Zenei

AU - Sasano, Hironobu

AU - Iino, Kazumi

AU - Mouri, Toraichi

N1 - Funding Information: We are grateful to K. Kikuchi for her excellent technical assistance. This study has been supported partly by a research grant for medical science from the Nippon University Alumni Association (to M. S.), a Grant-in-aid for Scientific Research (C; No. 09670117) from the Ministry of Education, Science, Sports and Culture of Japan (to K. T.), a Grant-in-aid for the Brain Science Research from the Ministry of Health and Welfare of Japan (to K. T.), and by the Gonryou Medical Foundation (to K. T.).

PY - 1998/4

Y1 - 1998/4

N2 - Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT- PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pons, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pons. Reverse phase high- performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain.

AB - Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT- PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pons, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pons. Reverse phase high- performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain.

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Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain

Abstract

Keywords

ASJC Scopus subject areas

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