Regional distribution of urocortin-like immunoreactivity and expression of urocortin mRNA in the human brain

Kazuhiro Takahashi, Kazuhito Totsune, Masahiko Sone, Osamu Murakami, Fumitoshi Satoh, Zenei Arihara, Hironobu Sasano, Kazumi Iino, Toraichi Mouri

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Regional distribution of urocortin-like immunoreactivity (UCN-LI) in the human brain was studied by radioimmunoassay and was compared with that of corticotropin-releasing hormone (CRH). In addition, the expression of UCN mRNA was examined by reverse transcriptase-polymerase chain reaction (RT- PCR) method. UCN-LI was detected in every region of brain examined, including hypothalamus, pons, cerebral cortex, and cerebellum. The concentrations of UCN-LI in the human brain were approximately 3 pmol/g wet weight in any brain region, and no marked regional difference was noted. On the other hand, the highest concentrations of CRH-LI were found in the frontal cortex, temporal cortex, and hypothalamus and the lowest in the pons. Reverse phase high- performance liquid chromatography of the UCN-LI in the human brain extract showed two immunoreactive peaks; one peak eluting earlier and one in the position of synthetic human UCN. RT-PCR showed that UCN mRNA was expressed in every region of brain examined. These findings indicated that UCN and UCN mRNA were widely expressed in the human brain.

Original languageEnglish
Pages (from-to)643-647
Number of pages5
JournalPeptides
Volume19
Issue number4
DOIs
Publication statusPublished - 1998 Apr

Keywords

  • Human brain
  • PCR
  • RT-PCR
  • Radioimmunoassay
  • Urocortin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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