Region-specific expression of mario reveals pivotal function of the anterior nondigit region on digit formation in chick wing bud

Takanori Amano, Koji Tamura

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We report the region-specific expression of a novel gene, named mario, whose expression domain is in the distal tip of the presumptive and developing digit 2 region in the developing chick wing bud. The anterior region-specific expression of mario corresponds well with the presence of digit 2, and fate map analysis showed that mario expression at early stages represents the presumptive digit 2 region. Using mario expression as a region-specific marker for the digit 2 region, several surgical operations were performed to obtain insights into digit 2 development in the chick wing. Cell fate tracing concomitant with a zone of polarizing activity (ZPA) implantation revealed that an additional digit 2 in the ZPA implantation into the anterior or middle region of wing bud is derived from the original digit 2 region (mario-positive region). Surgical manipulations revealed that the anterior nondigit region has an inhibitory effect on digit 2 formation. Taken together, these results suggest that the most-anterior region, including the anterior necrotic zone, restricts the position of digit 2 region by limiting the anterior border of the digit 2 region and preventing its expansion.

Original languageEnglish
Pages (from-to)326-336
Number of pages11
JournalDevelopmental Dynamics
Volume233
Issue number2
DOIs
Publication statusPublished - 2005 Jun

Keywords

  • Chick
  • Digit
  • Gene expression
  • Wing

ASJC Scopus subject areas

  • Developmental Biology

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