Region-Specific Activation of CRTC1-CREB Signaling Mediates Long-Term Fear Memory

Mio Nonaka, Ryang Kim, Hotaka Fukushima, Kazuki Sasaki, Kanzo Suzuki, Michiko Okamura, Yuichiro Ishii, Takashi Kawashima, Satoshi Kamijo, Sayaka Takemoto-Kimura, Hiroyuki Okuno, Satoshi Kida, Haruhiko Bito

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

CREB is a pivotal mediator of activity-regulated gene transcription that underlies memory formation and allocation. The contribution of a key CREB cofactor, CREB-regulated transcription coactivator 1 (CRTC1), has, however, remained elusive. Here we show that several constitutive kinase pathways and an activity-regulated phosphatase, calcineurin, converge to determine the nucleocytoplasmic shuttling of CRTC1. This, in turn, triggered an activity-dependent association of CRTC1 with CREB-dependent regulatory elements found on IEG promoters. Forced expression of nuclear CRTC1 in hippocampal neurons activated CREB-dependent transcription, and was sufficient to enhance contextual fear memory. Surprisingly, during contextual fear conditioning, we found evidence of nuclear recruitment of endogenous CRTC1 only in the basolateral amygdala, and not in the hippocampus. Consistently, CRTC1 knockdown in the amygdala, but not in the hippocampus, significantly attenuated fear memory. Thus, CRTC1 has a wide impact on CREB-dependent memory processes, but fine-tunes CREB output in a region-specific manner.

Original languageEnglish
Pages (from-to)92-106
Number of pages15
JournalNeuron
Volume84
Issue number1
DOIs
Publication statusPublished - 2014 Oct 1

ASJC Scopus subject areas

  • Neuroscience(all)

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