Regeneration of the gut requires retinoic acid in the budding ascidian Polyandrocarpa misakiensis

Nagamoto Kaneko, You Katsuyama, Kazuo Kawamura, Shigeki Fujiwara

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The protochordate ascidian Polyandrocarpa misakiensis has a striking ability to regenerate. When the posterior half of the adult body is amputated, the anterior half completely loses the esophagus, stomach and intestine. These organs are reconstituted in a week. Histological observation revealed that the regeneration involves transdifferentiation of the atrial epithelium near the cut surface. The morphological features of the gut primordium were similar to those observed in the developing bud of this species. Inhibitors of the synthesis of retinoic acid (RA) suppressed the formation of the gut. 13-cis RA rescued the regenerates from the inhibitor-induced hypoplasia. These results suggest that RA is required for the regeneration of the gut. A gene encoding the RA receptor (Pm-RAR) and its target gene, TRAMP, were expressed in and around the regenerating gut. Pm-RAR-specific and TRAMP-specific double-stranded RNA molecules inhibited the regeneration of the gut, indicating that the RA signal is mediated at least in part by Pm-RAR and TRAMP. These results suggested that RA triggers the transdifferentiation of the atrial epithelium into the gut in regenerating animals, as it does during asexual reproduction.

Original languageEnglish
Pages (from-to)457-468
Number of pages12
JournalDevelopment Growth and Differentiation
Volume52
Issue number5
DOIs
Publication statusPublished - 2010 Jun

Keywords

  • Ascidian
  • Regeneration
  • Retinoic acid
  • Transdifferentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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