Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth

Yasunori Kadowaki, Shunji Ishihara, Youichi Miyaoka, Mohammed Azharul Karim Rumi, Hiroshi Sato, Hideaki Kazumori, Kyoichi Adachi, Shin Takasawa, Hiroshi Okamoto, Tsutomu Chiba, Yoshikazu Kinoshita

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalFEBS Letters
Volume530
Issue number1-3
DOIs
Publication statusPublished - 2002 Oct 23

Keywords

  • Carcinogenesis
  • ERK1/2
  • Gastric cancer
  • Reg
  • Tyrosine kinase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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