Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth

Yasunori Kadowaki; Shunji Ishihara; Youichi Miyaoka; Mohammed Azharul Karim Rumi; Hiroshi Sato; Hideaki Kazumori; Kyoichi Adachi; Shin Takasawa; Hiroshi Okamoto; Tsutomu Chiba; Yoshikazu Kinoshita

doi:10.1016/S0014-5793(02)03398-7

Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth

Yasunori Kadowaki, Shunji Ishihara, Youichi Miyaoka, Mohammed Azharul Karim Rumi, Hiroshi Sato, Hideaki Kazumori, Kyoichi Adachi, Shin Takasawa, Hiroshi Okamoto, Tsutomu Chiba, Yoshikazu Kinoshita

Surgery

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.

Original language	English
Pages (from-to)	59-64
Number of pages	6
Journal	FEBS Letters
Volume	530
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)03398-7
Publication status	Published - 2002 Oct 23

Keywords

Carcinogenesis
ERK1/2
Gastric cancer
Reg
Tyrosine kinase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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Kadowaki, Y., Ishihara, S., Miyaoka, Y., Rumi, M. A. K., Sato, H., Kazumori, H., Adachi, K., Takasawa, S., Okamoto, H., Chiba, T., & Kinoshita, Y. (2002). Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth. FEBS Letters, 530(1-3), 59-64. https://doi.org/10.1016/S0014-5793(02)03398-7

Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth. / Kadowaki, Yasunori; Ishihara, Shunji; Miyaoka, Youichi; Rumi, Mohammed Azharul Karim; Sato, Hiroshi; Kazumori, Hideaki; Adachi, Kyoichi; Takasawa, Shin; Okamoto, Hiroshi; Chiba, Tsutomu; Kinoshita, Yoshikazu.

In: FEBS Letters, Vol. 530, No. 1-3, 23.10.2002, p. 59-64.

Research output: Contribution to journal › Article › peer-review

Kadowaki, Y, Ishihara, S, Miyaoka, Y, Rumi, MAK, Sato, H, Kazumori, H, Adachi, K, Takasawa, S, Okamoto, H, Chiba, T & Kinoshita, Y 2002, 'Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth', FEBS Letters, vol. 530, no. 1-3, pp. 59-64. https://doi.org/10.1016/S0014-5793(02)03398-7

Kadowaki, Yasunori ; Ishihara, Shunji ; Miyaoka, Youichi ; Rumi, Mohammed Azharul Karim ; Sato, Hiroshi ; Kazumori, Hideaki ; Adachi, Kyoichi ; Takasawa, Shin ; Okamoto, Hiroshi ; Chiba, Tsutomu ; Kinoshita, Yoshikazu. / Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth. In: FEBS Letters. 2002 ; Vol. 530, No. 1-3. pp. 59-64.

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abstract = "Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.",

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AU - Kadowaki, Yasunori

AU - Ishihara, Shunji

AU - Miyaoka, Youichi

AU - Rumi, Mohammed Azharul Karim

AU - Sato, Hiroshi

AU - Kazumori, Hideaki

AU - Adachi, Kyoichi

AU - Takasawa, Shin

AU - Okamoto, Hiroshi

AU - Chiba, Tsutomu

AU - Kinoshita, Yoshikazu

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AB - Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.

KW - Carcinogenesis

KW - ERK1/2

KW - Gastric cancer

KW - Reg

KW - Tyrosine kinase

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Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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