TY - JOUR
T1 - Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth
AU - Kadowaki, Yasunori
AU - Ishihara, Shunji
AU - Miyaoka, Youichi
AU - Rumi, Mohammed Azharul Karim
AU - Sato, Hiroshi
AU - Kazumori, Hideaki
AU - Adachi, Kyoichi
AU - Takasawa, Shin
AU - Okamoto, Hiroshi
AU - Chiba, Tsutomu
AU - Kinoshita, Yoshikazu
PY - 2002/10/23
Y1 - 2002/10/23
N2 - Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.
AB - Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.
KW - Carcinogenesis
KW - ERK1/2
KW - Gastric cancer
KW - Reg
KW - Tyrosine kinase
UR - http://www.scopus.com/inward/record.url?scp=18644386654&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18644386654&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(02)03398-7
DO - 10.1016/S0014-5793(02)03398-7
M3 - Article
C2 - 12387866
AN - SCOPUS:18644386654
VL - 530
SP - 59
EP - 64
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -