Reexpression of RAG-1 and RAG-2 genes in activated mature mouse B cells

Masaki Hikida, Masaharu Mori, Toshiyuki Takai, Ken Ichi Tomochika, Kiyohiro Hamatani, Hitoshi Ohmori

Research output: Contribution to journalArticlepeer-review

194 Citations (Scopus)

Abstract

Recombination activating genes (RAG-1 and RAG-2), involved in V(D)J rearrangement of immunoglobulin genes, have been thought to be expressed only in immature stages of B-cell development. However, RAG-1 and RAG-2 transcripts were found to be re-expressed in mature mouse B cells after culture with interleukin-4 in association with several different co-stimuli. Reexpression was also detected in draining lymph nodes from immunized mice. RAG-1 and RAG-2 proteins could be detected by immunofluorescence microscopy in the nuclei of B cells cultured in vitro and in the germinal centers of draining lymph nodes. These findings suggest that RAG gene products play a heretofore unsuspected role in mature B cells.

Original languageEnglish
Pages (from-to)2092-2094
Number of pages3
JournalScience
Volume274
Issue number5295
DOIs
Publication statusPublished - 1996 Dec 20

ASJC Scopus subject areas

  • General

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